Skip to main content
Log in

Study of light signal receptor ofStephanopyxis palmeriana during sexual reproduction

  • Biology
  • Published:
Chinese Journal of Oceanology and Limnology Aims and scope Submit manuscript

Abstract

We collected centric diatomStephanopyxis palmeriana samples in coastal waters of Xiamen for characteristic red light/far red light (R/FR) phytochrome reactions to identify its photoreceptor in the course of sexual reproduction. The result showed that pre-illumination of 2–3h red light before darkness could induce sexualization ofS. palmeriana, while the follow-up illumination of far red light could reverse the effect of red light, which is a featured reaction of phytochrome. The Southern Dot Blot was carried out to identify the type of phytochrome that induces the sexualization. The result also showed high homogeneity of DNA fragment ofS. palmeriana with phyB, but phyA. This means the photoreceptor in the process of sexual reproduction ofS. palmeriana is phytochrome B (phyB).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Borthwick, H. A., S. B. Hendricks, M. W. Parker, E. H. Toole and V. K. Toole, 1952. A reversible photoreaction controlling seed germination.Proceedings of the National Academy of Sciences of the United States of America 38: 662–666.

    Article  Google Scholar 

  • Dring, M. J., 1967. Phytochrome in red algaePorphyra tenera.Nature 215: 1 411–1 412.

    Article  Google Scholar 

  • Dring, M. J., 1988. Photocontrol of development in algae.Annual Review of Plant Physiology and Plant Molecular Biology 39: 157–174.

    Article  Google Scholar 

  • Flint, L. H. and E. D. McAlister, 1937. Wave lengths of radiation in the visable spectrum promoting the germination of light-sensitive lettuce seed.Smithsonian Miscellaneous Collections 96(2): 1–9.

    Google Scholar 

  • Guillard, R. R. L. and J. H. Rvther, 1962. Studies of marine planktonic diatoms. I.Cyclotella nana Hustedt andDetonula convervacea (Cleve)Gran.Can. J. Microbiol. 8: 229–239.

    Article  Google Scholar 

  • Hirschfeld, M., J. M. Tepperman, T. Clack, P. H. Quail and R. A. Sharrock, 1998. Coordination of phytochrome levels in phyB mutants ofArabidopsis as revealed by apoprotein-specific monoclonal antibodies.Genetics 149: 523–535.

    Google Scholar 

  • Hu, R., J. M. Lin, Q. Q. Lin, B. P. Han and T. L. Wang, 2003. Light effect and photo-receptor in the sexual reproduction process ofStephanopyxis palmeriana.Journal of Plant Physiology and Molecular Biology 29(1): 21–26. (in Chinese)

    Google Scholar 

  • Kidd, D. J. and J. C. Lagarias, 1990. Phytochrome from the green algaMesotaenium caldariorum.J. Biol. Chemistry 265(12): 7 029–7 035.

    Google Scholar 

  • Kircher, S., L. Kozma-Bognar, L. Kim, E. Adam, K. Harter, E. Schafer and F. Nagy, 1999. Light quality-dependent nuclear import of the plant photoreceptors phytochrome A and B.Plant Cell 11: 1 445–1 456.

    Article  Google Scholar 

  • Lüning, K., 2001. Cireadian growth inPorphyra umbilicalis (Rhodophyta): spectral sensitivity of the cireadian system.J. Phycol. 37: 52–58.

    Article  Google Scholar 

  • Quail, P. H., M. T. Boylan, B. M. Parks, T. W. Short, Y. Xu and D. Wagner, 1995. Phytochromes: hotosensory perception and Signal Transduction.Science 268: 675–680.

    Article  Google Scholar 

  • Reed, J. W., P. Nagpal, D. S. Poole, M. Furuya and J. Chory. 1993. Mutations in the gene for the red far-red light receptor phytochrome-B alter cell elongation and physiological-responses throughoutArabidopsis development.Plant Cell 5: 147–157.

    Article  Google Scholar 

  • Rudiger, W. and F. Thummler, 1991. Phytochrome, the visual pigment of plants.Angew. Chem. Int. Ed. Engl. 30: 1 216–1 228.

    Article  Google Scholar 

  • Sambrook, J. and D. W. Russell, 2001. Molecular Cloning: A laboratory manual 3rd ed. Cold Spring Harbor Laboratory Press. New York.

    Google Scholar 

  • Shacklock, P. S., N. D. Read and A. J. Trewavas, 1992. Cytosolic Free Calcium Mediates Red Light-Induced Photomorphogenesis.Nature 358: 753–755.

    Article  Google Scholar 

  • Smith, H., 2000. Phytochromes and light signal perception by plants—an emerging synthesis.Nature 407: 585–591.

    Article  Google Scholar 

  • Smith, H. and G. M. Jackson, 1987. Rapid Phytochrome Regulation of Wheat Seedling Extension—Light Pretreatment Extends Coupling Time, Increases Response Lag, and Decreases Light Sensitivity.Plant Physiology 84: 1 059–1 062.

    Google Scholar 

  • Somers, D. E., P. F. Devlin and S. A. Kay, 1998. Phytochromes and Cryptochromes and Crytochromes in the Entrainment of theArabidopsis Circadian Clock.Science 282: 1 488–1 490.

    Article  Google Scholar 

  • Tong, Z., Y. J. Zhao, T. Wang, N. H. Li and Y. Mawjuda, 2000. Photoreceptors and light-regulated development in plants.Acta Bot. Sin. 42(2): 111–115. (in Chinese)

    Google Scholar 

  • Yamaguchi, R., M. Nakamura, N. Mochizuki, S. A. Kay and A. Nagatani, 1999. Light-dependent translocation of a phytochrome B-GFP fusion protein to the nucleus in transgenicArabidopsis.Journal of Cell Biology 145: 437–445.

    Article  Google Scholar 

  • Yang, T. L., Z. H. Zuo, J. M. Lin, 2004. Effect of light and photo-receptors on the sexual reproduction process ofBidduphia sinensis.Marine Sciences 28(7): 44–48. (in Chinese)

    Google Scholar 

  • Winands, A. and G. Wagner, 1996. Phytochrome of the green algaMougeotia: cDNA sequence, autoregulation and phylogenetic position.Plant Mol. Biol. 32: 589–597.

    Article  Google Scholar 

  • Winslow, R. B. and A. O. Margaret, 2001. Photoreceptors in plant photomorphogenesis to date. Five phytochromes, two cryptochromes, one phototropin, and one superehromel.Plant Physiology 125: 85–88.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hu Ren.

Additional information

Supported by National Natural Science Foundation of China (No. 39670076)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ren, H., Junmin, L., Qiuqi, L. et al. Study of light signal receptor ofStephanopyxis palmeriana during sexual reproduction. Chin. J. Ocean. Limnol. 23, 330–334 (2005). https://doi.org/10.1007/BF02847156

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02847156

Key words

Navigation