Abstract
A potato leafroll virus (PLRV) isolate has been successfully transmitted to and recovered from two wild crucifers,Sisymbrium altissimum L. (Jim Hill or tumble mustard) andCapsella. bursa-pastoris (L.) Medic. (shepherd’s purse) by the green peach aphid (GPA),Myzus persicae (Sulzer). Virus antigen in both plant species was found to be higher in root tissue than in foliar tissue, based on enzyme-linked immunosorbent assay (ELISA) determinations.C. bursa-pastoris was apparently a relatively poorer source of inoculum for the GPA thanS. altissimum. Using two geographically-separated biotypes ofC. bursa-pastoris, a Washington biotype was found to contain higher antigen titer in both leaf and root tissue than a California biotype, as determined by ELISA. Field studies demonstrated that both weed species can serve as overwintering sources of PLRV
Compendio
Un aislamiento del virus del enrollamiento de la hoja de la papa (PLRV) ha sido exitosamente transmitido y recobrado de dos crucífieras silvestres,Sisymbrium altissimum L. (Jim Hill o mostaza postrada)y Capsella bursa-pastoris (L.) Medic, (boisa de pastor) por el áfido verde del melocotonero (GPA),Myzus persicae (Sulzer). Basándose en determinaciones efectuadas con la prueba inmunológica ELISA se encontró que el antígeno del virus en ambas especies vegetales era más alto en los tejidos de las raíces que en los de las hojas.C. bursa-pastoris fue aparentemente una fuente más o menos pobre de inóculo para el GPA, en comparación conS. altissimum. Utilizando dos biotipos geográficamente separados deC. bursa-pastoris, se encontró que un biotipo de Washington tenía, tanto en los tejidos de la hoja como en los de la raíz, una concentratión más alta de antígeno que un biotipo de California, tal como quedó comprobado con la técnica ELISA. Estudios de campo demostraron que ambas malezas pueden servir como fuentes de invernacion del PLRV.
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Fox, L., Biever, K.D., Toba, H.H. et al. Overwintering and monitoring of potato leafroll virus in some wild crucifers. American Potato Journal 70, 505–515 (1993). https://doi.org/10.1007/BF02846751
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DOI: https://doi.org/10.1007/BF02846751