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The influence of Fe2+ on growth and development of cells enzymatically isolated fromPorphyra yezoensis blades

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Abstract

Fe2+ acted as an accessorial factor for many cellular enzymatic reactions is very important for seaweed growth and development, but the Fe2+ requirement in nori had not been seen,Porphyra yezoensis cells were separated enzymatically and cultured in a series of sterilized seawater media containing various concentrations of Fe2+. The growth development and cell were investigated in this work. Through this experiment, two biologically-meant concentration scales were found, one is low concentrations, 12.1–102.1 μg/L, 10–100 times than that in seawater, favoring the development of isolated cells ofPorphyra and the other was high concentrations, more than 10mg/L inhibiting the cell growth, leading to the deformity and shrinkage of the cells. At the concentration of 50 mg/L, the cells stopped growing and died eventually.

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References

  • Boyer, G. L. and L. E. Brand, 1998. Trace Elements and Harmful Algal Blooms.In: Anderson, D. M., Cambella, A. D. and Hallegracff, G. M., eds, Physiological Ecology of Harmful Algal Blooms, Springer-Verlag Berlin Heidelberg p. 489–490.

    Google Scholar 

  • Byrne, R. H. and D. R. Kester, 1976. Solubility of hydrous ferric oxide and iron speciation in seawater.Mar. Chem. 4: 255–274.

    Article  Google Scholar 

  • Chen, L. C. M., 1986. Cell development ofPorphyra miniata (Rhodophyceae) under axenic culture.Bot. Mar. 29: 435–439.

    Google Scholar 

  • Chen, L. C. M., 1987. Protoplast morphyogenesis ofPorphyra leucosticta in culture.Bot. Mar. 30: 399–403.

    Article  Google Scholar 

  • Chen, L. C. M., 1989. Cell suspension culture fromPorphyra linearis (Rhodophyta), a multicellular marine red alga.J. Appl. Phycol. 1: 153–159.

    Article  Google Scholar 

  • Dai, J. and Z. Bao, 1988. Developmental studies on protoplasts ofPorphyra haitanensis.Chinese J. Genet. 15(3): 253–258. (in Chinese with English abstract)

    Google Scholar 

  • Dai, J., Z. Bao, Y. Tang and W. Liu, 1988. Studies on isolation ofPorphyra thallodic cells with enzymes and cell cultivation.Chinese J. Biotechnol. 4 (2): 127–133. (in Chinese with English abstract)

    Google Scholar 

  • Dai, J., Q. Zhang and Z. Bao, 1993. Genetic breeding and seedling raising experiments withPorphyra protoplasts.Aquaculture 111: 139–145. (in Chinese with English abstract)

    Article  Google Scholar 

  • Fang, Z., J. Dai, Y. Tang, W. Liu and Z. Bao, 1986. Isolation on the vegetative cells ofPorphyra yezoensis Ueda with enzymes and its application in aquaculture.Mar. Sci. 10 (3): 46–47. (in Chinese with English Abstract)

    Google Scholar 

  • Doucette, G. J. and P. J. Harrison, 1990. Some effects of iro and nitrogen stress on the red tide dinoflagellate.Mar. Ecol. Prog. Ser. 62: 293–306.

    Article  Google Scholar 

  • Freshwater, D. W. and D. F. Kapraun, 1989. Spheroplast isolation and propagation ofPorphyra carolinensis Coll et Cox for mariculture.Aquaculture 81: 101–110.

    Article  Google Scholar 

  • Gall, E. A., Y. M. Chiang and B. Kloareg, 1993. Isolation and regeneration of protoplasts fromPorphyra dentata andPorphyra crispata.Euro. J. Phycol. 28: 277–283.

    Article  Google Scholar 

  • Liu, W., Y. Tang, X. Liu and T. C. Fang, 1984. Studies on the preparation and on the properties of sea snail enzymes.Hydrobiologia 116/117: 319–320.

    Article  Google Scholar 

  • Lu, C. Q., 1983. Studies on vegetative cells of the thallus ofPorphyra I. The isolation and culture of vegetative cells, with observation on the development of isolated vegetative cell into Porphyra thallus.In: Chinese Phycological Society ed., Proceedings of the 1st Chinese Phycological Symposium. p. 45–55. (in Chinese with English Abstract)

  • Martin, J. H. and S. E. Fitzwater, 1988. Iron deficiency limits phytoplankton growth in the Northeast Pacific subarctic.Nature 331: 341–342.

    Article  Google Scholar 

  • Packer, M. A., 1994. Protoplast formation from single cells and small tissue fragments of wildPorphyra fronds (Rhodophyta).Bot. Mar. 37: 101–108.

    Article  Google Scholar 

  • Polne-Fuller, M. and A. Gibor, 1984. Developmental studies inPorphyra I. Blade differentiation inPorphyra perforata as expressed by morphology, enzymatic digestion and protoplast regeneration.J. Phycol. 20: 609–616.

    Article  Google Scholar 

  • Polne-Fuller, M. and A. Gibor, 1990. Developmental studies inPorphyra (Rhodophyceae) 3. Effect of culture conditions on wall regeneration and differentiation of protoplasts.J. Phycol. 26: 674–682.

    Article  Google Scholar 

  • Shen, S. and J. Dai, 2001. Isolated cells ofPorphyra yezoensis cultured on solid medium.Marine Science Bullitin 3(1): 45–50.

    Google Scholar 

  • Tang, Y., 1982. Isolation and cultivation of the vegetative cells and protoplasts ofPorphyra suborbiculata.J. Shandong Coll. Ocean. 12: 37–50. (in Chinese with English abstract)

    Google Scholar 

  • Wang, S., X. Zhang, Z. Xu and Y. Sun, 1986. A study on the cultivation of the vegetative cells and protoplasts ofP. haitanensis 1.Ocean. et Limno. Sinica 17(3): 217–221. (in Chinese with English abstract)

    Google Scholar 

  • Wang, S., Y. Sun, A. Lu and G. Wang, 1987. A study on the cultivation of the vegetative cells and protoplasts ofPorphyra haitanensis 2.Mar. Sci. 11(1): 1–7. (in Chinese with English abstract)

    Google Scholar 

  • Wells, M. L., 1991. The availability of iron in seawater: a perspection.Biol. Oceanogr. 6: 463–476.

    Google Scholar 

  • Yan, X. and S. Wang, 1993. Temperature, light and salinity influence to somatic cell development ofPorphyra haitanensis.Tropic Ocean 12 (1): 94–99. (in Chinese with English abstract)

    Google Scholar 

  • Zhao, H. and X. Zhang, 1981. Isolation and culture of the vegetative cells ofPorphyra yezoensis Ueda.J. Shandong Coll. Ocean. 11(1): 61–65. (in Chinese with English abstract)

    Google Scholar 

  • Zhu, M., X. Mu, R. Li and R. Lu, 2000. The effects of iron on growth, photosynthesis and biochemical composition of a marine algae Phaeodactylum tricornutum.Acta Oceanologica Sinica 22(1): 110–116. (in Chinese with English abstract)

    Google Scholar 

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This work was supported by the NSFC (No. 40206019).

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Songdong, S., Jixun, D. The influence of Fe2+ on growth and development of cells enzymatically isolated fromPorphyra yezoensis blades. Chin. J. Ocean. Limnol. 23, 48–53 (2005). https://doi.org/10.1007/BF02845143

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