Abstract
We examined the effects of polypeptide fromChlamys farreri (PCF) on the amount of hydroxyproline in guinea pig skin irradiated by chronic ultraviolet A (UVA) and ultraviolet B (UVB) radiation. PCF was applied locally before repeated exposure of guinea pig to UVA and UVB. The contents of hydroxyproline and other amino acids in guinea pig skin were determined by automatic amino acid analyzer. Our results showed that: (1) long-time UVA and UVB radiation can reduce dramatically the amounts of hydroxyproline, aspartic acid, threonine, glycine, phenylalanine and lysine in guinea pig skin in comparison with the control group (P<0.05); (2) Compared with model group, pre-treatment with 5% and 20% PCF prior to UVA and UVB radiation can inhibit the decline of amino acids content in guinea pig skin in a dose-dependent manner (P<0.05). As the decrease of hydroxyproline, glycine and lysine contents in the skin directly reflexes type I collagen degeneration, our results indicated that the chronic application of PCF can protect skin type I collagen against UV radiation, and thus protect skin from photoaging.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bissett, D. L., D. P. Hannon, and T. V. Orr, 1987. An animal model of solar-aged skin: histological, physical, and visible changes in UV irradiated hairless mouse skin.Photochem Photobiol. 46: 367–378.
Bissett, D. L., R. Chatterjee and D. P. Hannon, 1990. Photoprotective effect of topical anti-inflammatory agents against ultraviolet radiation-induced chronic skin damage in the hairless mouse.Photodermatol. Photoimmunol. Photomed. 7: 153–158.
Differy, B. D., 1987. Analysis of the risk of skin cancer from sunlight and solaria in subjects living in Northern Europe.Photodermatology 4: 118–126.
Eriko, S., F. Manabu, H. Minoru et al., 2002. CD19- dependent B lymphocyte signaling thresholds influence skin fibrosis and autoimmunity in the tight-skin mouse.J. Clin. Invest. 11: 1453–1462.
Halliday, G. M., B. O. Robertson and R. C. Barnetson, 2000. Topical retinoic acid enhances, and a dark tan protects, from subedemal solar-simulated photocarcinogenesis.J. Invest. Dermatol. 114: 923–927.
Masataka, S., T. Yoshitaka, K. Naomichi et al., 2001. Complete primary structure of rainbow trout type I collagen consisting of α1 (I) α2 (I) α3 (I) heterotrimers.Eur. J. Biochem. 268: 2817–2827.
Prockop, D. J., K. I. Kivirikko, 1995. Collagens: molecular biology, diseases, and potentials for therapy.Annu. Rev. Biochem. 64: 403–34.
Tornaletti, S. and G. P. Pfeifer, 1996. UV damage and repair mechanisms in mammalian cells.Bioessay 18: 221–232.
Uitto, J., 1979. Collagen polymorphism. Isolation and partial characterization of α1 (I) trimer molecules in normal human skin.Arch. Biochem. Biophys. 192: 371–379.
Urbach, F., 1989. Potential effects of altered solar ultraviolet radiation on human skin cancer.Protochem. Photobiol. 50: 507–513.
Wang, C. B., M. O. He, S. Z. Qin et al., 1998. Antioxidative effect of polypeptide fromChlamys farreri in vitro.Chinese Journal of Marine Drugs 3: 15–17.
Wang, C. B., R. Y. Yao, Z. T. Liu et al., 2002. Protective effects of polypeptide fromChlamys farreri on hairless mice damaged by ultraviolet A.Acta Pharmacol. Sin. 9: 813–818.
Yao, R. Y. and C. B. Wang, 2002. Protective effect of polypeptide fromChlamys farreri on HeLa cells damaged by ultraviolet A.Acta Pharmacol. Sin. 11: 1018–1022.
Author information
Authors and Affiliations
Corresponding author
Additional information
Project No. 39970638 supported by the NSFC, and also supported by the Science and Technology Bureau of Qingdao (No: 2001-28-50).
Rights and permissions
About this article
Cite this article
Guoying, Y., Pengli, C., Kun, G. et al. Effects of polypeptide fromChlamys farreri on amino acid content in guinea pig skin irradiated by chronic ultraviolet A and B. Chin. J. Ocean. Limnol. 22, 440–442 (2004). https://doi.org/10.1007/BF02843643
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02843643