Abstract
DNA polymorphisms were evaluated for the eight strains representingTilletia controversa Kuhn,T. laevis Kuhn andT. tritici (Bjerk.) Winter using polymorase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. DNA regions coding for the internal transcribed spacers (ITS) were amplified and digested with five four-base restriction enzymes (Msp I,Hinf I,Taq I,Hha I andHae III). No differences were shown in the PCR-RFLP patterns among the strains tested. This indicatedT. controversa, T. laevis andT. tritici are very closely related.
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This study was supported in part by systematic Mycology & Liohenology Laboratory of Sciance Institute of China
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Chengliang, G., Dongsheng, C. & Desheng, Z. RFLPs in internal transcribed spacers of ribosomal DNA from three species ofTilletia . J. Northeast For. Univ. 7, 29–31 (1996). https://doi.org/10.1007/BF02843051
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DOI: https://doi.org/10.1007/BF02843051