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Genetic differentiation withinEriochoir sinensis (milne, edwards) revealed by allozyme and RAPD

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Abstract

We analyzed 17 allozymes, and 20 primers in order to detect the genetic differentiation between commercial populations (Changjiang River, Liaohe River) ofEriochoir sinensis. Ten allozymes (LDH, MDH, ME, IDH, EST, ALP, AAT, CTL, POD, SOD) showed 21 loci by vertically discontinuos buffer system polyacrylamide gel electrophoresis. RAPD profiles generated by 12 ten-base primers showed 63 loci. The percentage of polymorphic loci and the expected heterozygosity obtained by using allozyme analysis were lower than those obtained by RAPD. The index of similarity between these two populations were 0.955 and 0.932 as revealed by allozyme analysis and RAPD technology. There was gene flow between the above populations.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Acdemy of Sciences, 266071, Qingdao, China

    Cui Zhao-xia, Xiang Jian-hai, Song Lin-sheng & Zhou Ling-hua

  2. Dalian Fisheries College, 116023, Dalian, China

    Shi Wei-liang

Authors
  1. Cui Zhao-xia
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  2. Xiang Jian-hai
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  3. Song Lin-sheng
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  4. Zhou Ling-hua
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  5. Shi Wei-liang
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Additional information

Contribution No. 4025 from the Institute of Oceanology and Limnology, Chinese Academy of Sciences.

Project 39470141 supported by NSFC. Work also supported by Climbing Program B (PDB 6-5-3) and No. 863 High-technology Program 863-819-01-05.

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Zhao-xia, C., Jian-hai, X., Lin-sheng, S. et al. Genetic differentiation withinEriochoir sinensis (milne, edwards) revealed by allozyme and RAPD. Chin. J. Ocean. Limnol. 18, 234–240 (2000). https://doi.org/10.1007/BF02842669

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  • DOI: https://doi.org/10.1007/BF02842669

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