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The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallopChlamys farreri

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Abstract

To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallopChlamys farreri population. Thirteen enzymes (MDH, ME, IDH, GPI, PGM, PEP−LG, PEP−PP, ACP, AK, PK, AAT, SOD, EST) in three buffer systems (TC, Ph6.9; TMME, Ph 7.4; and EBT, pH8.9) were selected and 22 loci were used for the analysis, among them 7 loci (Gpi, Pgm, Pep−LG-1, Pep−PP Aat-2, Est-2, Est-3) were polymorphic which attributed 31.82% to the total. The average of heterozygosity was 0.113 and most of the studied loci showed heterozygote deficiencies. The same specimens were investigated using 10 arbitrarily selected primers (10-base). Twenty two of 54 RAPD fragments were polymorphic with average heterozygosity of 0.194. The result indicated that the two types of markers reflected a consistent trend in the parameter values of genetic diversity of the population, but RAPD revealed more information of genetic variation than allozyme electrophoresis.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China

    Liu Baozhong, You Feng, Dong Bo & Xiang Jianhai

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  1. Liu Baozhong
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  2. You Feng
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  3. Dong Bo
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  4. Xiang Jianhai
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Correspondence to Xiang Jianhai.

Additional information

Supported by Chinese Basic Research Project (G1999012007) and National Natural Science Foundation of China (No. 39700017).

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Baozhong, L., Feng, Y., Bo, D. et al. The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallopChlamys farreri . Chin. J. Ocean. Limnol. 24, 295–299 (2006). https://doi.org/10.1007/BF02842631

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  • DOI: https://doi.org/10.1007/BF02842631

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