Abstract
In this study, the inhibitory effect of jujuboside A (JuA) on a penicillin sodium (Na-PCN) induced hyperactivity model was investigated. Cortical EEG (electroencephalogram) and the concentration of hippocampal Glutamate (Glu) were monitored simultaneously in vivo as indicators of rat's excitatory state. Power spectral density (PSD) and gravity frequency of PSD were calculated. JuA (0.05 g/L and 0.1 g/L) inhibited the EEG excitation effect caused by Na-PCN by increasing the power of δ1 and δ2 bands (P<0.01 vs model) and lowering the gravity frequency of PSD (P<0.01 vs model). JuA also remarkably reduced the Glu elevation induced by Na-PCN (P<0.05 vs model). Diazepam also depressed Glu concentration and lowered the gravity frequency, but it showed a different EEG pattern in increased β2-activity (P<0.01 vs model). EEG excitation caused by Na-PCN correlated with Glu elevation during the first hour. Neurophysiological inhibitory effects of JuA and diazepam were more persistent than their Glu inhibitory effects.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Begley, D.J., Reichel, A., Ermisch, A., 1994. Simple high-performance liquid chromatographic analysis of free primary amino acid concentrations in rats plasma and cisternal cerebrospinal fluid.J Chromatogr B,657: 185–191.
Bradford, H.F., 1995. Glutamate, GABA and epilepsy.Prog. Neurobiol,47:477–511.
Chen, X.C., Sheng, C., Zheng, X.X., 2001. Direct nitric oxide imaging in cultured hippocampal neurons with diamino-anthraquinone and confocal microscopy.Cell Biol Int,25:593–598.
El-Yamany, N.A., Horn, E., 2002. Time courses of aspartate and glutamate concentrations in the focus area during penicillin sodium induced epileptiform activity in awake rats.Arch Ital Biol,140:13–30.
Fiacro, J., Francisco, V., Jose, C.R., Francisco, E.V., Marcos, V., Hector, P., 1999. Seizures induced by penicillin microinjections in the mesencephalic tegmentum.Epilepsy Research,38:33–44.
Futamachi, K.J., Prince, D.A., 1975. Effects of penicillin on an excitatory synapse.Brain Research,100:589–597.
Horn, E., Esseling, K., 1993. Arrest of seizure series induced by an intracortical injection of penicillin in the awake rat.Pharm Biochem Behav,45:857–863.
Horn, E., Esseling, K., Wagner, R., 1991. Time course of interictal EEG patterns induced by a penicillin injection into the olfactory cortex.Pharmacol Biochem Behav,40:351–357.
Kozhechkin, S.N., 2003. Comparative electroencephalographic evaluation of anxiolytics afobazol and diazepam in MR and MNRA inbred rats with different anxiety levels.Eksp Klin Farmakol,66:38–41.
Marijtje, L.A.J., Clementina, M.R., Jan, E., Willie, J.S., Anke, S., Anton, M.L.C., 2000. The influence of diazepam on the electroencephalogram evoked potential interrelation in rats.Neurosci Lett,293:83–86.
Ribak, C.E., 1987. Epilepsy hypothesis.Science,238:1292–1293.
Shou, C.H., Wang, J., Zheng, X.X., Guo, D.W., 2001. Inhibitory effects of Jujuboside A on penicillin sodium induced hyperactivity in rat hippocampal CA1 area in vitro.Acta Pharmacol Sin,22:986–990.
Shou, C.H., Feng, Z.Y., Wang, J., Zheng, X.X., 2002. The inhibitory effects of jujuboside A on rat hippocampus in vivo and in vitro.Planta Med,68:799–803.
Soderpalm, B., 2002. Anticonvulsants: aspects of their mechanisms of action.Eur J Pain,6(Suppl A): 3–9.
Su, S., Zhang, H.Y., Zheng, X.X., 2003. Sorting the sleep stages of rat based on the gravity frequency and complexity measure.Chinese Pharamcol Bulletin,19: 949–953 (in Chinese).
Ticku, M.K., 1983. Benzodiazepine-GABA receptor-ionophore complex, current concepts.Neuropharmacol,22:1459–1470.
Uysal, H., Kuli, P., Caglar, S., Inan, L.E., Akarsu, E.S., Palaoglu, O., Ayhan, I.H., 1996. Antiseizure activity of insulin: insulin inhibits pentylenetetrazole, penicillin and kainic acid-induced seizures in rats.Epilepsy Research,25:185–190.
Ye, W.L., 1988. High performance liquid chromatographic determination of amino acids in brain by precolumn derivatization with o-phthaldialdehyde.Acta Physiologica Sin,40:308–313.
Zhang, M., Ning, G.M., Shou, C.H., Lu, Y.J., Hong, D.H., Zheng, X.X., 2003. Inhibitory effect of jujuboside A on glutamate-mediated excitatory signal pathway in hippocampus.Planta Med,69:692–695.
Author information
Authors and Affiliations
Additional information
Project supported by the National Natural Science Foundation of China (No. 30170275), and the Key Laboratory for Biomedical Engineering of the Ministry of Education of China, and Science and Technology Department of Zhejiang Province (No. 011106239), China
Rights and permissions
About this article
Cite this article
Ying-jun, L., Jun, Z., Shao-min, Z. et al. Inhibitory effects of jujuboside A on EEG and hippocampal glutamate in hyperactive rat. J Zheijang Univ Sci B 6, 265–271 (2005). https://doi.org/10.1007/BF02842463
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02842463
Key words
- Jujuboside A (JuA)
- Diazepam
- Glutamate (Glu)
- Electroencephalogram (EEG)
- Hyperactivity
- Penicillin sodium (Na-PCN)
- Power spectral density (PSD)
- Gravity frequency