Abstract
Objective: To determine the protective effect of monosialoganglionside (GM1) and evaluate the influence of GMI on expression of N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in Sprague-Dawley (SD) rats with focal cerebral ischemia-reperfusion (I/R). Methods: Left middle cerebral artery (MCA) was occluded by an intraluminal suture for 1 h and the brain was reperfused for 72 h in SD rats when infarct volume was measured. GM1 (10 mg/kg) was givenip (intraperitoneally) at 5 min (group A), 1 h (group B) and 2h (group C) after MCA occlusion (MCAo). Expression of NMDAR1 was detected by Western blot at various time after reperfusion (4 h, 6 h, 24 h, 48 h and 72 h) in ischemic hemispheres of the rats with or without GM1 administered. Results: (1) Adjusted relative infarct volumes of groups A and B were significantly smaller than that of group C and the control group (P<0.01, andP<0.05, respectively). (2) Expression level of NMDAR1 was temporally high at 6 h after reperfusion, and dipped below the normal level at 72 h after reperfusion. GM1 at 5 min after MCAo significantly suppressed the expression of NMDAR1 at 6 h after reperfusion (P<0.05 vs the control). At 72 h after reperfusion, the NMDAR1 expression level of rats treated with GM1 administered (at 5 min or 2 h after MCAo) was significantly higher than that of the control (P<0.05). Conclusion: GM1 can time-dependently reduce infarct volume in rats with focal cerebral I/R partly through stabilizing the expression of NMDAR1.
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Project (No. 2004QN012) supported by the Youth Talent Special Fund of Health Bureau of Zhejiang Province, the National Basic Research Program (973) of China (No. G1999054000) and the National Natural Science Foundation of China (No. 30371637)
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Jian-ren, L., Mei-ping, D., Er-qing, W. et al. GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat. J Zheijang Univ Sci B 6, 254–258 (2005). https://doi.org/10.1007/BF02842461
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DOI: https://doi.org/10.1007/BF02842461