Abstract
Objective: To find new potential biomarkers and establish the patterns for the detection of ovarian cancer. Methods: Sixty one serum samples including 32 ovarian cancer patients and 29 healthy people were detected by surface-enhanced laser desorption/ionization mass spectrometry (SELDI-MS). The protein fingerprint data were analyzed by bioinformatics tools. Ten folds cross-validation support vector machine (SVM) was used to establish the diagnostic pattern. Results: Five potential biomarkers were found (2085 Da, 5881 Da, 7564 Da, 9422 Da, 6044 Da), combined with which the diagnostic pattern separated the ovarian cancer from the healthy samples with a sensitivity of 96.7%, a specificity of 96.7% and a positive predictive value of 96.7%. Conclusions: The combination of SELDI with bioinformatics tools could find new biomarkers and establish patterns with high sensitivity and specificity for the detection of ovarian cancer.
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Project (No. G1998051200) supported by the National Basic Research Program (973) of China
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Jie-kai, Y., Shu, Z., Yong, T. et al. An integrated approach utilizing proteomics and bioinformatics to detect ovarian cancer. J Zheijang Univ Sci B 6, 227–231 (2005). https://doi.org/10.1007/BF02842456
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DOI: https://doi.org/10.1007/BF02842456