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Active changes of lignification-related enzymes in pepper response toGlomus intraradices and/orPhytophthora capsici

  • Plant & Animal Sciences and Biotechnology
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Abstract

The activities of enzymes responsible for lignification in pepper, pre-inoculation with arbuscular mycorrhizal (AM) fungus ofGlomus intraradices and/or infection with pathogenic strain ofPhytophthora capsici, and the biological control effect ofG. intraradices on Phytophthora blight in pepper were investigated. The experiment was carried out with four treatments: (1) plants pre-inoculated withG. intraradices (Gi), (2) plants pre-inoculated withG. intraradices and then infected withP. capsici (Gi+Pc). (3) plants infected withP. capsici (Pc), and (4) plants without any of the two microorganisms (C). Mycorrhizal colonization rate was reduced by about 10% in pathogen challenged plants. Root mortality caused by infection ofP. capsici was completely eliminated by pre-inoculation with antagonisticG. intraradices. On the ninth day after pathogen infection, Peroxidase (POD) activity increased by 116.9% in Pc-treated roots but by only 21.2% in Gi+Pc-treated roots, compared with the control, respectively. Polyphenol oxidase (PPO) and Phenylalanine ammonia-lyase (PAL) activities gradually increased during the first 3 d and dramatically decreased in Pc-treated roots but slightly decreased in Gi+Pc-treated roots, respectively. On the ninth day after pathogen infection, PPO and PAL decreased by 62.8% and 73.9% in Pc-treated roots but by only 19.8% and 19.5% in Gi+Pc-treated roots, compared with the control, respectively. Three major POD isozymes (45000, 53000 and 114000) were present in Pc-treated roots, while two major bands (53000 and 114000) and one minor band (45000) were present in spectra of Gi+Pc-treated roots, the 45000 POD isozyme was significantly suppressed byG. intraradices, suggesting that the 45000 POD isozyme was induced by the pathogen infection but not induced by the antagonisticG. intraradices. A 60000 PPO isozyme was induced in Pc-treated roots but not induced in Gi+Pc-treated roots. All these results showed the inoculation of antagonisticG. intraradices alleviates root mortality, activates changes of lignification-related enzymes and induces some of the isozymes in pepper plants infected byP. capsici. The results suggested thatG. intraradices is a potentially effective protection agent againstP. capsici.

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Authors and Affiliations

  1. Division of Biological and Environmental Chemistry, School of Agriculture and Life Science, Chonnam National University, 500-757, Gwangju, Korea

    Zheng Hu-zhe & Kim Kil Yong

  2. Department of Engineering, Liaoning Agricultural Vocation-Technical College, 115009, Yingkou, China

    Zheng Hu-zhe, Cui Chun-lan, Zhang Yu-ting & Jing Yu

  3. Department of Forestry, Shenyang Agricultural University, 110161, Shenyang, China

    Wang Dan

Authors
  1. Zheng Hu-zhe
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  2. Cui Chun-lan
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  3. Zhang Yu-ting
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  4. Wang Dan
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  5. Jing Yu
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  6. Kim Kil Yong
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Project supported by Korea Science and Engineering Foundation (KOSEF) through the Agricultural Plants Stress Research Center (APSRC) at Chonnam National University, Korea

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Hu-zhe, Z., Chun-lan, C., Yu-ting, Z. et al. Active changes of lignification-related enzymes in pepper response toGlomus intraradices and/orPhytophthora capsici . J. Zheijang Univ.-Sci. B 6, 778–786 (2005). https://doi.org/10.1007/BF02842437

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  • DOI: https://doi.org/10.1007/BF02842437

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