Abstract
The difference of sensitivity to indole-3-acetic acid (IAA) combined with horseradish peroxidase (HRP) in K562 and BXPC-3 cells was investigated. The cell proliferation was determined by MTT assay. The cell cycle and apoptosis of K562 and BXPC-3 cells were examined by a fluorescence flow cytometer (FCM) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) respectively. The experimental results show that IAA and HRP could inhibit BXPC-3 cell proliferation greatly compared with K562 cell during the first 48 h. The cell cycle was arrested predominantly at G2/M phase in K562 and BXPC-3 cells. The cell apoptosis of K562 and BXPC-3 was induced by IAA/HRP. There was a significant difference between the two cell lines since BXPC-3 cells were more sensitive than K562 cells by treatments with combination of IAA and HRP.
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Funded by the National Natural Science Foundation of China (No. 30170011), the Construction Fund for “211” Project of the Ministry of Education of China and the Natural Science Foundation of Hubei Province (No. 2006ABA197)
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Yali, B., Deli, L., Dali, Z. et al. The difference of sensitivity between BXPC-3 and K562 cells by treatments with combination of indole-3-acetic acid and horseradish peroxidase. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 21, 95–98 (2006). https://doi.org/10.1007/BF02841214
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DOI: https://doi.org/10.1007/BF02841214