Abstract
Objective: To investigate the immunological function of a yeast expression system for thymosin α1 (Tα1). Methods: A constructed Tα1 yeast expression system was used to investigate the immunological function of orally administered Tα1. Dried yeast containing three different concentration of Tα1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Tα1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4+ and CD8+ levels were detected by flow cytometry assay. TNF-α, IFN-γ, IL-2, IL-6 and IL-10 levels were detected by liquid chip. Results: In normal Balb/c mice or immune inhibition Balb/c mice, CD8+ levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8+ levels in synthesized Tα1 group (18.77%±4.72%), small dose group (13.48%±6.17%) and large dose group (22.74%±1.09%) were significantly higher than that in empty yeast control group (7.49%±2.14%). Conclusion: Orally administered Tα1 has its certain immunomodulatory function.
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Andreone, P., Cursaro, C., Gramenzi, A., Margotti, M., Ferri, E., Talarico, S., Biselli, M., Felline, F., Tuthill, C., Martins, E., Gasbarrini, G., Bernardi, M., 2001. In vitro effect of thymosin-alpha1 and interferon-alpha on Th1 and Th2 cytokine synthesis in patients with chronic hepatitis C.J. Viral. Hepat.,8(3):194–201.
Garaci, E., Pica, F., Rasi, G., Favalli, C., 2000. Thymosin alpha 1 in the treatment of cancer: From basic research to clinical application.Int. J. Immunopharmacol.,22(12): 1067–1076.
Kullavanuaya, P., Treeprasertsuk, S., Thong-Ngam, D., Chaermthai, K., Gonlachanvit, S., Suwanagool, P., 2001. The combined treatment of interferon alpha-2a and thymosin alpha 1 for chronic hepatitis C: the 48 weeks end of treatment results.J. Med. Assoc. Thai,84(Suppl 1): S462–468.
Saruc, M., Ozden, N., Turkel, N., Ayhan, S., Hock, L.M., Tuzcuoglu, I., Yuccyar, H., 2003. Long-term outcomes of thymosin-alpha 1 and interferon alpha-2b combination therapy in patients with hepatitis B e antigen (HBeAg) negative chronic hepatitis B.J. Pharm. Sci.,92(7): 1386–1395.
Zavaglia, C., Airoldi, A., Pinzello, G., 2000. Antiviral therapy of HBV- and HCV-induced liver cirrhosis.J. Clin. Gastroenterol.,30(3):234–241.
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Project (No. 2003C13015) supported by the Key Project from Science and Technology Department of Zhejiang Province, China
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Xiang-ming, C., Han-liang, J., Lin-fu, Z. et al. Immunomodulatory function of orally administered thymosin α1. J. Zheijang Univ.-Sci. B 6, 873–876 (2005). https://doi.org/10.1007/BF02840996
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DOI: https://doi.org/10.1007/BF02840996