Abstract
High performance liquid chromatography with a C18 reverse-phase column was used to separate the five components in cough syrup, including acetaminophen, p-aminophenol, caffeine, chlorphenamine maleate and guaifenesin. The mobile phase consists of 15wt% acetonitrile, 0.004mol/L sodium heptyl sulfonate, 0.03 mole/L potassium di-hydrogen phosphate and triethylamine (volume ratio 13:40:44:3), the pH of which is adjusted to 3.0 by phosphoric acid. The contents of the five components are analyzed on an ultraviolet spectrophotometer at 254nm, with a flow rate of 0.4 mL/min. The results show that the calibration curves are linear in a certain range. The average recovery of five components is between 96.31% and 102.3%.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Zhang Liqing, Tang Xi Zhu Xian-liang, The Simultaneous Determination of Five Domponents including Acetaminophen by Ridge Regression Spectrophotometry.Journal of Wuhan University of Technology—Mater. Sci. Ed, 2001, 16(2): 79
Zhang Liqing, Wu Xiaohua, Tang Xi Zhu Xian-liang, Simultaneous Spectrophotometric Determination of Five-Components in Cough Syrup by Partial Least Squares.Journal of Analytical Science, 2002, 18(4): 318
Zhang Liqing, Wu Xiaohua, Tang Xi Zhu Xian-liang, Research into Simultaneous Spectrophotometric Determination of Components in Cough Syrup by Principal Component Regression.Spectroscopy and Spectral Analysis, 2002, 22(3): 427
Baty J D, Lindsay R M, Sharp Sheila. Use of High-performance Liquid Chromatography in the Measurement of in vitro Acetylation.J. Chromatogr, 1986; 353: 329–37
Brega A, Prandini P, Amagliiet al. Determination of phenol,m-, o- andp-cresol,p-aminophenol andp-nitrophenol in Urine by High-performance Liquid Chromatography.J Chromatogr, 1990; 535(1–2): 311–16
Jain R, Bhatia A. Thin Layer Chromatographic Separation of Some Sulfa Drugs Using Acetylacetone as a Coupling Agent.J Indian Chem Soc, 1990; 67(7): 616–17
Spell J C, Stewart J T. Quantitative Analysis ofp-aminosalicylic Acid and Its Major Impuritym-aminophenol inp-aminosalicylic.J Planar Chromatogr, 1994; 7(6): 472–3
Author information
Authors and Affiliations
Additional information
ZHANG Li-qing: Born in 1965.
Financially supported the Science Foundation of Analysis Test of Zhejiang Province (No. 03 084)
Rights and permissions
About this article
Cite this article
Li-qing, Z., Xiao-hua, W., Ying, L. et al. Simultaneous determination of five components including acetaminophen by reversed-phase high performance liquid chromatography. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 19, 34–35 (2004). https://doi.org/10.1007/BF02838358
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02838358