Abstract
Objective: To observe the changes of coagulation and fibrinolysis function in human umbilical cord vein endothelial cells (HUVEC) induced by recombinant human tumor necrosis factor-α (rhTNF-α) and the effect of Naotaifang extract (NTE) on it.Methods: Cultured HUVEC is randomly divided into six groups: Control group, NTE control group (only 2 g/L NTE), rhTNF-α group (100 μg/ L rhTNF-α), and low-dosage, middle-dosage and high-dosage NTE group (100 μg/L rhTNF-α and 0. 67 g/L, 2 g/L, 6 g/L NTE). The coagulation activity of frozen-dissolved HUVEC, von Willebrand factor (vWF) content in the conditioned medium and tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor (PAD activity were to be detected after 24 hrs.Results: Compared with the control group, PAI activity were enhanced, vWF release markedly increased in conditioned medium of TNF-α group (P < 0.01) and the frozen-dissolved HUVEC markedly shortens the rabbit plasma prothrombin time, and the above changes could be significantly inhibited by the 3 dosages of NTE (P < 0.05,P < 0. 01).Conclusion: NTE is effective in inhibiting the coagulation activity of the HUVEC non-stimulated or stimulated by rhTNF-α to enhance the vWF release, and to adjust fibrinolytic function, and mainly to inhibit the PAI activity.
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The item is supported by Natural Science Foundation of Hunan Province (No. 98JJY 2027) and Young-middle Aged Science and Technological Fund of Hunan Province (NO. 00JZYZ145)
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Jin-wen, G., Song, C. & Hui-bin, Z. Effect of Naotaifang extract on changes of coagulation in human umbilical cord vein endothelial cell and fibrinolysis function induced by recombinant human Tumor Necrosis Factor-α. Chin. J. Integr. Med. 9, 219–223 (2003). https://doi.org/10.1007/BF02838039
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DOI: https://doi.org/10.1007/BF02838039