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Expression of TGF-β2 in LECs of age-related nuclear, cortex cataract and the relationship among TGF-β2, proliferation, apoptosis and transdifferentiation

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Wuhan University Journal of Natural Sciences

Abstract

To detect the pathogenesis of age-related cataract, we analyzed the expression of TGF-β2 mRNA, proliferation cell nuclear antigen (PCNA), Bcl-2/Bax, fibronection (FN), vimentin protein and the density of lens epithelial cells(LECs) of nuclear cataract, cortex cataract and normal LECs. Results showed that the expression level of TGF-β2, mRNA, FN and vimentin protein was higher in LECs of cortex cataract than that of nuclear cataract and normal lens. But the level of Bcl-2/Bax and PCNA was on the contrary. The density of LECs was (4250.63±275.05)/mm2 in cortex cataract. It was (5438.40±262.30)/mm2 in nuclear cataract, and (5368.63±211.07)/mm2 in normal LECs resepectively. There was significant difference between cortex and nuclear cataract (p<0.05). These suggested that TGF-β2 might take an important part in the process of age-related cataract. Age-related nuclear cataract was related to the proliferation LECs. While cortex cataract was related to the apoptosis and transdifferentiation of LECs.

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Authors and Affiliations

  1. Ophthalmology Department, Zhongnan Hospital, Wuhan University, 430071, Wuhan, Hubei, China

    Ye Lin, Cai Xiaojun, Luo Hong & Deng Ping

  2. Medical School, Wuhan University, 430071, Wuhan, Hubei, China

    Zhang Baifang

Authors
  1. Ye Lin
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  2. Cai Xiaojun
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  3. Zhang Baifang
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  4. Luo Hong
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  5. Deng Ping
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Corresponding author

Correspondence to Zhang Baifang.

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Foundation item: Supported by the Natural Science Foundation of Hubei Province (2000-J059)

Biography: YE Lin(1973-), female, Ph. D. candidate, research direction: pathogenesis of cataract.

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Lin, Y., Xiaojun, C., Baifang, Z. et al. Expression of TGF-β2 in LECs of age-related nuclear, cortex cataract and the relationship among TGF-β2, proliferation, apoptosis and transdifferentiation. Wuhan Univ. J. Nat. Sci. 11, 756–760 (2006). https://doi.org/10.1007/BF02836703

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  • DOI: https://doi.org/10.1007/BF02836703

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