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A new rat model of cerebral infarction based on the injury of vascular endothelial cell

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Abstract

Objective: A new rat model of cerebral infarction was developed to elucidate the contribution of vascular endothelial cell during focal cerebral infarction formation.Methods: Forty-eight Sprague-Dawley (SD) rats were randomly divided into the model group, sham operation group, and control group for indexes observation of triphenyltetrazolium chloride (TTC) dyeing, neurological deficit, plasma tissue-type plasminogen activator (tPA) activity, plasminogen activator inhibitor (PAD activity, thromboxane B2(TXB2) content, and 6-keto-prostaglandin (6-keto-PGF1α) content.Results: (1) The highest neurological score appeared at 6 h after operation, descending significantly at sequential time. (2) Using TTC dyeing and optical microscope technique, pathological changes in brains were observed. (3) Compared with control group and sham operation groups, there was a decrease in tPA activity of model rats at the initial 12 h after injection of sodium laurate (P<0. 05), PAI activity decreased markedly in the model group at 24 h after injection of sodium laurate. (4) In plasma TXB2 concentration reached the highest level compared at 6 h after injection of sodium laurate, but there were not obvious differences in plasma 6-keto-PGF1α, concentration among all groups (P> 0. 05).Conclusion: Focal cerebral infarction in rats could be induced by some sodium laurate, showing ischemic cerebrum necrosis, function disorder of vascular endothelium-platelet, fibrinolysis abnormality. This model could play an important role in researching the contribution of vascular endothelial cell during cerebral infarction development, preventing and curing by traditional Chinese medicine.

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Authors and Affiliations

  1. Department of Physiology, Hunan University of Traditional Chinese Medicine, 410007, Changsha

    Chen Yi & Deng Bing-xiang

  2. Department of Science and Technology, Hunan University of Traditional Chinese Medicine, 410007, Changsha

    Ge Jin-wen

Authors
  1. Chen Yi
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  2. Ge Jin-wen
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  3. Deng Bing-xiang
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Correspondence to Ge Jin-wen.

Additional information

Supported by Young-middle Aged Science and Technological Fund of Hunan Province (No. 00JZY2145)

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Yi, C., Jin-wen, G. & Bing-xiang, D. A new rat model of cerebral infarction based on the injury of vascular endothelial cell. Chin. J. Integr. Med. 11, 195–200 (2005). https://doi.org/10.1007/BF02836504

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  • DOI: https://doi.org/10.1007/BF02836504

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