Skip to main content

Fatty acid composition of ethanolamine phosphoglycerides in different areas of the gerbil brain after chronic exposure to trichloroethylene

Neurochemical Pathology volume 3, Article number: 151 (1985) Cite this article

Abstract

Exposure of Mongolian gerbils to trichloroethylene (TCE) (320 ppm) in an inhalation chamber continuously for 3 mo resulted in an altered fatty acid pattern of phospholipid in discrete areas of the brain.

Lipids were extracted from four brain regions: the cerebral cortex, the hippocampus, the cerebellar vermis posterior, and the brain stem. No changes induced by TCE were found in lipid class distribution among the different regions examined. Whole brain weights and weights of the dissected pieces were also unchanged. In ethanolamine phosphoglycerides from the cerebral cortex and the hippocampus, a decrease was found among long-chain fatty acids derived from linolenic acid with a corresponding increase of the linoleic acid family. The cerebellar vermis and the brain stem were less affected.

Since areas rich in gray matter were affected more than those with a high proportion of white matter, it seems reasonable to assume that the fatty acid alterations of ethanolamine phosphoglycerides occur mainly in the gray matter. Furthermore, we suggest that the alterations can be a compensatory mechanism for the membrane fluidizing properties of TCE.

This is a preview of subscription content, access via your institution.

References

  • Bartlett G. R. (1959) Phosphorus assay in column chromatography.J. Biol. Chem. 243, 466–468.

    Google Scholar 

  • Benjamins J. A. and Smith M. E. (1977) Metabolism of myelin, inMyelin (Morell P., ed.) pp. 233–270. Plenum Press, NY

    Google Scholar 

  • Bergman K. (1979) Whole body autoradiography and allied tracer techniques in distribution and elimination studies of some organic solvents. Benzene, toluene, xylene, styrene, methylene chloride, chloroform, carbon tetrachloride and trichloroethylene.Scand. J. Work Environ. Health 5, Suppl. 1.

    Google Scholar 

  • Bonse G., Urban T., Reichert D., and Henchler D. (1975) Chemical reactivity, metabolic oxirane formation and biological reactivity of chlorinated ethylenes in the isolated perfused rat liver preparation.Biochem. Pharmacol. 24, 1829–1834.

    PubMed  Article  CAS  Google Scholar 

  • Chin J. H. and Goldstein D. B. (1977) Drug tolerance in bio-membranes: A spin label study of the effects of ethanol.Science 196, 684–685.

    PubMed  Article  CAS  Google Scholar 

  • Franley R. J. and Amador E. (1968) Serum cholesterol measurement based on ethanol extraction and ferric chloride-sulfuric acid.Clin. Chim. Acta 21, 255–263.

    Article  Google Scholar 

  • Guengerich F. P., Wang P., and Davidson N. K. (1982) Estimation of isozymes of microsomal p450 in rats, rabbits and humans using immunochemical staining coupled with sodium dodecyl sulfate-polyacrylamide gel electrophoresis.Biochemistry 21, 1698–1706.

    PubMed  Article  CAS  Google Scholar 

  • Haglid K. G., Briving C., Hansson H.-A., Kjellstrand P., Rosengren L., Stavrou D., Swedin U., and Wronski A. (1981) Trichlorethylene: Long-lasting changes in the brain after rehabilitation.Neurotoxicology 2, (4) 659–673.

    PubMed  CAS  Google Scholar 

  • Haglid K. G., Kjellstrand P., Rosengren L., Wronski A., and Briving C. (1980) Effects of trichloroethylene inhalation on proteins of the gerbil brain.Arch. Toxicol. 43, 187–199.

    PubMed  Article  CAS  Google Scholar 

  • Kjellstrand P., Lanke J., Bjerkemo M., Zetterqvist L., and M»nsson L. (1980) Irreversible effects of trichlorethylene exposure on the central nervous system.Scand. J. Work Environ. Health 6, 40–47.

    PubMed  CAS  Google Scholar 

  • Kyrklund T., Alling C., Haglid K. G., and Kjellstrand P. (1983) Chronic exposure to trichloroethylene: Lipid and acyl group composition in the gerbil cerebral cortex and hippocampus.Neurotoxicology 4(4), 35–42.

    PubMed  CAS  Google Scholar 

  • Kyrklund T., Goracci G., Haglid K. G., Rosengren L., Porcellati G., and Kjellstrand P. (1984a) Chronic effects of trichloroethylene upon S-100 protein content and lipid composition in gerbil cerebellum.Scand. J. Work Environ. Health 10, 89–93.

    PubMed  CAS  Google Scholar 

  • Kyrklund T., Alling C., Kjellstrand P., and Haglid K. G. (1984b) Chronic effects of perchloroethylene on the composition of lipid and acyl groups in the cerebral cortex and hippocampus of the gerbil.Toxicology Lett. 22(3), 343–349.

    Article  CAS  Google Scholar 

  • Le Blanc A. E. and Kalant H. (1975) Ethanol-induced cross tolerance to several homologous alcohols in the rat.Toxicol. Appl. Pharmacol. 32, 123–128.

    Article  Google Scholar 

  • Reddy T. S., Rajalakshmi R., and Ramakrishnan C. V. (1982) Effects of nutritional rehabilitation on the content and lipid composition of brain gray and white matter of neonatally undernourished rats.J. Neurochem. 39(5) 1297–1301.

    PubMed  Article  CAS  Google Scholar 

  • Reynolds E. S. and Moslen M. T. (1977) Damage to hepatic cellular membranes by chlorinated olefins with emphasis on synergism and antagonism.Environ. Health Perspect. 21, 137–147.

    PubMed  Article  CAS  Google Scholar 

  • Richards C. D. and White A. E. (1975) The actions of volatile anaesthetics on synaptic transmission in the dentate gyrus.J. Physiol. 252, 244–257.

    Google Scholar 

  • Seeman P. (1972) The membrane actions of anesthetics and tranquilizers.Pharmacol. Rev. 24, 583–655.

    PubMed  CAS  Google Scholar 

  • Svennerholm L. and Fredman P. (1980) A procedure for the quantitative isolation of brain gangliosides.Biochim. Biophys. Acta 617, 97–109.

    PubMed  CAS  Google Scholar 

  • Svennerholm L. (1968) Distribution and fatty acid composition of phosphoglycerides in normal human brain.J. Lipid Res. 9, 570–579.

    PubMed  CAS  Google Scholar 

  • Waters E. M., Gerstner H. B., and Huff J. E. (1977) Trichloroethylene. 1. An overview.J. Toxicol. Environ. Health. 2, 671–707.

    PubMed  CAS  Article  Google Scholar 

Download references

Author information

Affiliations

  1. Institute of Neurobiology, University of Göteborg, Sweden

    Titus Kyrklund & Kenneth G. Haglid

  2. Department of Psychiatry and Neurochemistry, University of Lund, Sweden

    Christer Alling

  3. Department of Zoophysiology, University of Lund, Sweden

    Per Kjellstrand

Authors
  1. Titus Kyrklund
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christer Alling
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Per Kjellstrand
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kenneth G. Haglid
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kyrklund, T., Alling, C., Kjellstrand, P. et al. Fatty acid composition of ethanolamine phosphoglycerides in different areas of the gerbil brain after chronic exposure to trichloroethylene. Neurochemical Pathology 3, 151 (1985). https://doi.org/10.1007/BF02834267

Download citation

  • DOI: https://doi.org/10.1007/BF02834267

Index Entries

  • Brain stem
  • cerebral cortex
  • chronic exposure, of brains to trichlorethylene
  • fatty acid composition
  • gerbil brain, exposure to trichloroethylene
  • hippocampus, exposure to trichlorethylene
  • lipid composition, and exposure to trichlorethylene in brain tissue
  • phospholipids, and exposure to trichlorethylene in brain tissue
  • trichlorethylene, and brain fatty acids
  • vermis posterior, and trichlorethylene exposure