Abstract
In the brain of quaking and shiverer mutants, vitamin E content was normal when related to both wet weight and dry weight. When related to lipid extract, phosphorus, and polyunsaturated fatty acids, vitamin E was slightly increased only in the quaking mutant. In the sciatic nerve from trembler mutants, vitamin E was 134% of control values in the dry material, but normal in relation to wet weight. It was 260% in the lipid extract and 716% based on phosphorus. In relation to total fatty acids, there was a threefold increase in trembler mutants. Interestingly, it was increased approximately three times when related to 18∶2 n−6, 20∶4 n−6, and 20∶5 n−3, and seven times when related to 22∶6 n−3. The fact that the amount of vitamin E in fresh weight was normal, suggests that vitamin E plays a role in some nonmembrane material, such as the extracellular matrix or the basal lamina.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baumann N., Jacque C. M., Pollet S., and Harpin M. L. (1968) Fatty acid and lipid composition of the brain of a myelin deficient mutant, the “Quaking” mouse.Eur. J. Biochem. 4, 340–344.
Baumann N. (ed.) (1980) Neurological mutation affecting myelination.INSERM, Symposium n 014, Elsevier.
Bieri J., Tolliver T. J., and Catignani G. L. (1979) Simultaneous determination of α-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography.Am. J. Clin. Nutr. 32, 2143–2149.
Bourre J. M., Boiron F., Boutry J. M., Cassagne C., Chanez C., Darriet D., Dothi A., Dumont O., Flexor M. A., Hauw J. J., Koenig H., Larrouquère S., Piciotti M., and Trouillet V. (1984a) Biochemical aspects of the trembler mouse (dysmyelinating mutant of the peripheral nervous system with onion bulb proliferation).Neuromuscular Diseases, (Serratrice G. et al., eds.) Raven, New York.
Bourre J. M., Pascal G., Durand G., Masson M., Dumont O., and Piciotti, M. (1984b) Alterations in the fatty acid composition of rat brain cells (neurons, astrocytes and oligodendrocytes) and of sub-cellular fractions (myelin and synaptosomes) induced by a diet devoid of n−3 fatty acids.J. Neurochem. 42, 342–348.
Chen P. S., Toribara T. Y., and Warner H. (1956) Microdetermination of phosphorus,Analyt. Chem. 28, 1756–1758.
Demopoulos H. B., Flamm E. S., Selgman M. L., Mitamauir J. A., and Ransohoff J. (1979) Membrane perturbations in central nervous system injury: Theoretical basis for free radical damage and a review of the experimental data, inNeural Trama (Popp J., Bourke R. S., Nelson L. R., and Kimelberg H. K., eds.) pp. 63–78, Raven, New York.
Dinesen B. and Clausen J. (1976) The influence of vitamin E deficiency and combined deficiency in vitamin E and polyunsaturated fatty acids on the biosynthesis and degradation of rat central nervous system myelin.Nutr. Metabol. 20, 95–111.
Diplock A. T. (1983) The role of vitamin E in biological membranes, inBiology of vitamin E. Ciba Foundation symposium 101, pp. 45–55. Pitman, London.
Folch J., Lees M., and Sloane-Stanley G. H. (1957) Simple method for the isolation and purification of total lipides from animal tissues.J. Biol. Chem.,226, 497–509.
Giasuddin A. S. M. and Diplock A. T. (1981) The influence of vitamin E on membrane lipids of mouse fibroblasts in culture.Arch. Biochem. Biophys. 210, 348–362.
Halliwell B. and Gutteridge M. L. (1985) Oxygen radicals and the nervous system.Trends Neurosci. 22–26.
Hogan E. C and Greenfield S. (1984) Animal models of genetic disorders of myelin, inMyelin, 2nd Ed. pp. 489–534, Elsevier.
Larrouquère-Regnier S., Boiron F., Darriet D., Cassagne C., and Bourre J. M. (1979) Lipid composition of sciatic nerve from dysmyelinating trembler mouse.Neurosci. Lett. 15, 135–139.
Lowry O. H., Rosebrough N. J., Farr A. L., and Randall R. J. (1951) Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265–275.
Morrison W. R. and Smith L. M. (1964) Preparation of fatty acid methyl esters and dimethyl-acetals from lipids with boron fluoride-methanol.J. Lipid Res. 5, 600–608.
Muller D. P. R., Lloyd J. K., and Wolff O. H. (1983)Nutrition: The changing scene. Vitamin E and neurological function.Lancet, 225–228.
Murphy D. J. and Mavis R. D. (1981) Membrane transfer of α-tocopherol. Influence of soluble α-tocopherol binding factors from the liver, lung, heart, and brain of the rat.J. Biol. Chem. 256, 10464–10468.
Nelson J. (1983) Neuropathological studies of chronic vitamin E deficiency in mammals including humans, inBiology of vitamin E. Ciba Foundation Symposium 101, pp. 92–105. Pitman, London.
Norton W. T. and Poduslo S. E. (1973) Myelination in rat brain: method of myelin isolation.J. Neurochem. 21, 749–757.
Pollet S., Ermidou S., Le Saux F., Monge M., and Baumann N. (1978) Microanalysis of brain lipids: multiple two dimensional thin-layer chromatography.J. Lipid Res. 19, 916–921.
Radin N. S. (1981) Extraction of tissue lipids with a solvent of low toxicity.Methods Enzymol. 72, 5–11.
Tappel A. L. (1980) Measurement of and protection from in vivo lipid peroxidation, inFree radicals in biology. (Pryor W. A., ed.) vol. 4, pp. 1–47, Academic, New York.
Vatassery G. T., Berry J. F., Younozai R., and Lam Bergad, P. (1976) Effect of vitamin E deficiency on the lipid class and fatty acid composition of rat brain gray and white matter.Lipids 11, 317–321.
Vatassery G. T., Angerhofer C. K., and Knox C. A. (1984a) Effect of age on vitamin E concentrations in various regions of the brain and a few selected peripheral tissues of the rat, and on the uptake of radioactive vitamin E by various regions of rat brain.J. Neurochem. 43, 409–412.
Vatassery G. T., Angerhofer C. K., Knox C. A., and Deshmukh D. S. (1984b) Concentrations of vitamin E in various neuroanatomical regions and subcellular fractions, and the uptake of vitamin E by specific areas, of rat brain.Biochim. Biophys. Acta 792, 118–122.
Vitiello F. and Zanetta J. P. (1978) Thin-layer chromatography of phospholipids.J. Chromatogr. 166, 637–640.
Westerberg E., Friberg M., and Akesson B. (1981) Assay of brain tocophorols using high performance liquid chromatography.J. Liquid Chromatot. 4, 109–121.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bourre, J.M., Clement, M. & Chaudiere, J. Alteration of the α-tocopherol content in the brain and peripheral nervous tissue of dysmyelinating mutants. Neurochemical Pathology 7, 91–97 (1987). https://doi.org/10.1007/BF02834211
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02834211