Abstract
On the basis of conventional enzyme-linked immunosorbent assay (ELISA) and bienzyme substrate recycle, ELISA-bienzyme colorimetric substrate recycle was developed in the present study. The sensitivity of this method increased 15 times than that of ELISA for the measurement of Tau and increased 55 times forp-Tau. The linear detective rang of this method expanded 3 times higher than that of conventional ELISA for Tau and had the same as ELISA forp-Tau. So, ELISA-bienzyme colorimetric substrate recycle could be used to detect alnormally phosphorylated tau in cerebrospinal fluid.
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Foundation item: Supported by the National Natural Science Foundation of China (39925012, 301000057) and 973 Research Project of China (2006CB500703)
Biography: DUAN Qui-hong (1972-), female, Ph. D., research direction: neurology.
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Qiu-hong, D., Xiao-chuan, W., Xi-ming, W. et al. Development of an ultrasensitive ELISA-bienzyme colorimetric substrate recycle assay for measurement of Tau. Wuhan Univ. J. Nat. Sci. 10, 1047–1050 (2005). https://doi.org/10.1007/BF02832465
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DOI: https://doi.org/10.1007/BF02832465