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Heterologous expression of amylase gene fromSaccharomycopsis fibuligera in an industrial strain ofSaccharomyces cerevisiae

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Wuhan University Journal of Natural Sciences

Abstract

An α-amylase encoding gene was amplified by polymerase chain reaction fromSaccharomycopsis fibuligera and inserted into a shuttle vector YEp352, together with the yeast phosphoglycerate kinase 1 promoter and α-factor signal gene. The recombinant expression plasmid pLA8α was transformed into an industrial strain ofSaccharomyces cerevisiae Sc-11. The activity of the α-amylase produced by the transformant Sc-11-pLA8α was 6.3 U/mL and the starch utilization rate in YPS medium was 42%. The purified amylase was analyzed by SDS-PAGE, showing a molecular weight of 55×103 protein band. Furthermore, the residual sugar, ethanol and some volatile compounds in the fermented worts under simulating brewing conditions were determined by chromatographic analyses. The fermentation characteristics of Sc-11-pLA8α were similar to that of Sc-11 and only minor changes in the concentration of flavor compounds could be observed.

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Authors and Affiliations

  1. Life Science College, Sichuan University, 610064, Chengdu, Sichuan, China

    Liu Zeng-ran & Zhang Guang-yi

  2. Bioscience and Bioengineering College, Hebei Economices and Business University, 050061, Shijazhuang, Hebei, China

    Liu Zeng-ran, Long Zhang-fu & Liu Shi-gui

Authors
  1. Liu Zeng-ran
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  2. Zhang Guang-yi
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  3. Long Zhang-fu
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  4. Liu Shi-gui
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Corresponding author

Correspondence to Zhang Guang-yi.

Additional information

Foundation item: Supported by the National Tenth Five-Year Hi-Technique Project (2001BA708B05-04).

Biography: LIU Zeng-ran (1964-), fenale, Ph. D., research, direction: food and biotechnology.

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Zeng-ran, L., Guang-yi, Z., Zhang-fu, L. et al. Heterologous expression of amylase gene fromSaccharomycopsis fibuligera in an industrial strain ofSaccharomyces cerevisiae . Wuhan Univ. J. Nat. Sci. 10, 1041–1046 (2005). https://doi.org/10.1007/BF02832464

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  • DOI: https://doi.org/10.1007/BF02832464

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