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Refined procedures for accurate determination of solution structures of nucleic acids by two dimensional nuclear magnetic resonance spectroscopy

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Abstract

New procedures have been described for accurate determination of solution structures of nucleic acids. These are two fold; new two dimensional nuclear magnetic resonance techniques and better approaches for interpretation of nuclear magnetic resonance data for structure determination purposes. The significant development in two dimensional nuclear magnetic resonance techniques for this purpose areω 1 -scaling and recording of pure phase spectra. Use ofω 1-scaled correlated and nuclear Overhauser effect spectra for estimation of interproton distances and 1H-1H coupling constants has been described. Computer simulation procedures for exact determination of structure have been described. Experimental spectra demonstrating the application of new procedures have been presented.

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Abbreviations

2D NMR:

Two-dimensional nuclear magnetic resonance spectroscopy

COSY:

correlated spectroscopy

NOE:

nuclear Overhauser effect

NOESY:

NOE spectroscopy

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Authors and Affiliations

  1. Chemical Physics Group, Tata Insgtitute of Fundamental Research, Homi Bhabha Road, 400 005, Bombay, India

    R. V. Hosur, K. V. R. Chary, A. Sheth, Girjesh Govil & H. T. Miles

  2. National Institute of Health, 20892, Bethesda, Maryland, USA

    H. T. Miles

Authors
  1. R. V. Hosur
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  2. K. V. R. Chary
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  3. A. Sheth
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  4. Girjesh Govil
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  5. H. T. Miles
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Hosur, R.V., Chary, K.V.R., Sheth, A. et al. Refined procedures for accurate determination of solution structures of nucleic acids by two dimensional nuclear magnetic resonance spectroscopy. J Biosci 13, 71–86 (1988). https://doi.org/10.1007/BF02832214

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