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Device for miniscale isoelectric focusing of proteins

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Abstract

A simple device is developed for mini-scale electrofocusing of proteins. The main apparatus consists of only two glass tubes joined by a small tubing. No special cooling system, stopcocks, stands, etc., are needed. Even the need for a peristaltic pump for fractionation is eliminated. The apparatus does not require very high voltages and the amount of Ampholines is drastically reduced. The model can be used for analytical as well as semi-quantitative purposes.

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Authors and Affiliations

  1. Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Poona, India

    H. S. Pawar, A. M. Bodhe, M. V. Rele & H. G. Vartak

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  1. H. S. Pawar
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  2. A. M. Bodhe
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  3. M. V. Rele
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  4. H. G. Vartak
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Pawar, H.S., Bodhe, A.M., Rele, M.V. et al. Device for miniscale isoelectric focusing of proteins. J Biosci 13, 43–46 (1988). https://doi.org/10.1007/BF02832211

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  • DOI: https://doi.org/10.1007/BF02832211

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