Skip to main content
SpringerLink
Log in

Application of an optical biosensor to study the interaction between porphyrins and wheat germ agglutinin

  • Published:
Wuhan University Journal of Natural Sciences

Abstract

An optical biosensor with a stirred cuvette has been used to monitor the interaction of immobilized wheat germ agglutinin (WGA) with two water-soluble cationic porphyrins. The association constants (K a) of the free base porphyrin and its Zn(II) complex form were 2.66 and 27.31×105 L/mol at 20°C respectively. The interactions of the free base porphyrin were further investigated at temperatures between 15°C and 37°C. The thermodynamics parameters, changes in free energy, enthalpy and entropy, were −31.23, 22.92, 54.15 kJ/mol respectively. The heat capacity change was −355.53 J·mol−1·K−1. The binding was driven by entropic contribution, and showed strong enthalpy-entropy compensation. It was governed primarily by hydrophobic forces.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bonnett R. Photosensitizers of the Porphyrin and Phtalocyanine Series for Photodynamic Therapy [J].Chem Soc Rev, 1995,24: 19–33.

    Article  Google Scholar 

  2. Dougherty T J, Gomer C J, Henderson B W,et al. Photodynamic Therapy [J].J Natl Cancer Inst, 1998,90(12): 889–905.

    Article  Google Scholar 

  3. Wang Lei, Zhu Xunjin, Fu Shitao,et al. Synthesis of Water-Soluble Metalloporphyrins and Their Inhibiting Action on the Growth Metabolism ofE. coli [J].Chem J Chin Univ, 2003,24(7): 1145–1149 (Ch).

    Google Scholar 

  4. Tan A M, Xu B, Huang S Q,et al. Thermochemical Study on the Growth Metabolism of Human Promyelocytic Leukemia HL-60 Cells Inhibited by Water-Soluble Metalloporphyrins [J].Thermochim Acta, 1999,333: 99–102.

    Article  Google Scholar 

  5. Kreimer-Birnbaum M. Modified Porphyrins, Chlorins, Phthalocyanines, and Purpurins: Second-Generation Photosensitizers for Photodynamic Therapy [J].Semin Hematol, 1989,26(2): 157–173.

    Google Scholar 

  6. Wirth M, Fuchs A, Wolf M,et al. Lectin-Mediated Drug Targeting: Preparation, Binding Characteristics, and Antiproliferative Activity of Wheat Germ Agglutinin Conjugated Doxorubicin on Caco-2 Cells [J].Pharm Res, 1998,15(7): 1031–1037.

    Article  Google Scholar 

  7. Gabor F, Kausegger U, Wrth M. The Interaction between Wheat Germ Agglutinin and Other Plant Lectins with Prostate Cancer Cells Du-145 [J].Int J Pharm, 2001,221(1–2): 35–47.

    Article  Google Scholar 

  8. Kenoth R, Reddy D R, Maiya B G,et al. Thermodynamic and Kinetic Analysis of Porphyrin Binding to Trichosanthes Cucumerina Seed Lectin [J].Eur J Biochem, 2001,268 (21): 5541–5549.

    Article  Google Scholar 

  9. Sultan N A, Maiya B G, Swamy M J. Thermodynamic Analysis of Porphyrin Binding to Momordica Charantia (Bitter Gourd) Lectin [J].Eur J Biochem, 2004,271(15): 3274–3282.

    Article  Google Scholar 

  10. Leatherbarrow R J, Edwards P R. Analysis of Molecular Recognition Using Optical Biosensors [J].Cur Opin Chem Boil, 1999,3(5): 544–547.

    Article  Google Scholar 

  11. Zeng X X, Nakaaki Y, Murata T,et al. Chemoenzymatic Synthesis of Glycopolypeptides Carrying Alpha-Neu5Ac (2→3)-beta-D-Gal-(1→3)-alpha-D-GalNAc, Beta-D-Gal-(1→3)-alpha-D-GalNAc, and Related Compounds and Analysis of Their Specific Interactions with Lectins [J].Arch Biochem Biophys, 2000,383(1): 28–37.

    Article  Google Scholar 

  12. Davila J, Harriman A. Photoreactions of Macrocyclic Dyes Bound to Human Serum Albumin [J].Photochem Photobiol, 1990,51(1): 9–19.

    Article  Google Scholar 

  13. Komath S S, Kenoth R, Giribabu L,et al. Fluorescence and Absorption Spectroscopic Studies on the Interaction of Porphyrins with Snake Gourd (Trichosanthes anguina) Seed Lectin [J].J Photochem Photobiol B: Biol, 2000,55(1): 49–55.

    Article  Google Scholar 

  14. Ross P D, Subramanian S. Thermodynamics of Protein Association Reactions. Forces Contributing to Stability [J].Biochem, 1981,20(11): 3096–3102.

    Article  Google Scholar 

  15. Sturtevant J M. Heat Capacity and Entropy Changes in Processes Involving Proteins [J].Proc Natl Acad Sci USA, 1977,74: 2236–2240.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, China

    Qin Yimin, Pan Nina & Zou Guolin

Authors
  1. Qin Yimin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pan Nina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zou Guolin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zou Guolin.

Additional information

Foundation item: Supported by the National Natural Science Foundation of China (30370366)

Biography: QIN Yimin (1976-), female, Ph. D., research direction: applied biochemistry and molecular biology.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yimin, Q., Nina, P. & Guolin, Z. Application of an optical biosensor to study the interaction between porphyrins and wheat germ agglutinin. Wuhan Univ. J. Nat. Sci. 11, 432–436 (2006). https://doi.org/10.1007/BF02832138

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02832138

Key words

CLC number

Search

Navigation