Summary
The transfection efficiency of oligonucleotide and plasmid to the HL-60 cell line with lipofectaminedPLUS was compared through observing the transfection rate and the expression duration of exogenous gene in the target cells. The results showed that the transfection rate of oligonucleotide to the HL-60 was about 90%–95% and it had no obvious attenuation within 84h. However, the plasmid transfection rate was only 5%–25% and it was decreased significantly within 60h. It was suggested that the transfection of oligonucleotide with liposomes was better than that of plasmid.
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Tang Yi, male, born in 1970, Doctor in charge
This project was supported by a grant from National Natural Sciences Foundation of China (No. 39800149).
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Yi, T., Wenli, L., Jianfeng, Z. et al. Transfection efficiency comparison of oligonucleotide and plasmid to the HL-60 cell line with liposomes. Current Medical Science 25, 24–25 (2005). https://doi.org/10.1007/BF02831377
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DOI: https://doi.org/10.1007/BF02831377