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Antagonistic effects of trasilast on proliferation and collagen synthesis induced by TGF-\sB2 in cultured human trabecular meshwork cellsin cultured human trabecular meshwork cells

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Summary

Whether tranilast had antagonistic effect on proliferation inhibition and collagen synthesis promotion induced by TGF-\sB2 in cultured human trabecular meshwork cells was investigated. Suspension of 1×104 cultured human trabecular meshwork cells of 3–5 passage was distributed in each well of a 96-well disk and divided into control group and experimental group. After 24 h, 0 μg/ml (control), 12.5 μg/ml, 25 μg/ml, 50μg/ml tranilast with 3.2 ng/ml TGF-\sB2 were added into the incubation medium. Another 24 h later, proliferation and collagen synthesis in cultured human trabecular meshwork cells were examined respectively by using tetrazolium-based semiautomated colormetric (MTT) assay and3H-proline incorporation with liquid scintillation technique. The results showed absorbance (A) values of the experimental groups were 0.9036±0.3017, 1.1361±0.1352, 1.2457±0.1524 according to the different concentrations of tranilast, and 0.8956±0.1903 of the control group. In comparison with the control group, 25 μg/ml (q=3.23,P<0.05), 50 μg/ml (q′=4.70,P<0.01) tranilast significantly antagonized the decrease of theA values induced by TGF-\sB2 in the cultured human trabecular meshowrk cells. In comparison with the control group [817.37±124.21 cpm/104 cells], 12.5 μg/ml (620.33±80.46 cpm/104 cells,q′=4.26,P<0.05), 25 μg/ml (59.4.58±88.13 cpm/104 cells,q′=4.81,P<0.01), 50 μg/ml (418.64±67.90 cpm/104 cells,q′=8.62,P<0.01) tranilast significantly inhibited the incorporation of3H-proline into the cultured human trabecular meshwork cells promoted by TGF-\sB2 in a dose-dependent manner. It was concluded that tranilast had the antagonistic effect on the proliferation inhibition and collagen synthesis promotion induced by TGF-\sB2 in the cultured human trabecular meshwork cells.

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Authors and Affiliations

  1. Department of Emergency, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China

    Da Banghong

  2. Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China

    Cao Yang & Wei Houren

  3. Department of Ophthalmology, Hanchuan TCM Hospital, 432300, Hanchuan, China

    Chen Zhixin

  4. Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, 63110, St. Louis, USA

    Shui Yinbo

  5. Department of Anatomy, Tongji Medical College of Huazhong, University of Science and Technology, 430030, Wuhan, China

    Li Zhongyu

Authors
  1. Da Banghong
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  2. Cao Yang
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  3. Wei Houren
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  4. Chen Zhixin
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  5. Shui Yinbo
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  6. Li Zhongyu
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Additional information

Da Banghong, female, born in 1973, Resident.

This project was supported by a grant from the National Natural Sciences Foundation of China (No. 38970758).

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Banghong, D., Yang, C., Houren, W. et al. Antagonistic effects of trasilast on proliferation and collagen synthesis induced by TGF-\sB2 in cultured human trabecular meshwork cellsin cultured human trabecular meshwork cells. Current Medical Science 24, 490–492 (2004). https://doi.org/10.1007/BF02831117

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  • DOI: https://doi.org/10.1007/BF02831117

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