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Effects of retinoic acid on the β-catenin/TCF pathway in culture porcine tracheobronchial epithelial cells

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The effects of retinoic acid on the β-catenin/TCF pathway in cultured porcine tracheo-bronchial epithelial cells (TBEC) were investigated. After TBEC were treated with retinoic acid at various concentrations, mRNA and protein changes of β-catenin in cytoplasm, nucleus and whole cell of the TBEC were observed by immunocytochemical stain, RT-PCR and Western blotting. And the changes of the target gene cyclinD1 of β-catenin/TCF pathway were also observed. It was found that there was no significant difference in β-cat mRNA level after retinoic acid treatment. However, the expression of β-catenin in the whole cell and cytoplasm was elevated with the increase of retinoic acid concentration (P<0.01). The nuclear protein β-catenin and target gene cyclinD1 of β-catenin/TCF pathway was decreased (P<0.05). It was indicated that retinoic acid could increase β-catenin level of the whole cell protein and decreased nuclear β-catenin, downregulating β-cat/TCF signaling activity and reducing target gene cyclinD1 protein level. As a result, retinoic acid can downregulate β-catenin/TCF pathway in porcine tracheobronchial epithelial cell, suggesting that retinoic acid can inhibit the proliferation and accelerate differentiation of tracheobronchial epithelial cells.

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Li Yuan, female, born in 1977, Assistan:

This project was supported by a grant from National Natural Sciences Foundation of China for youth scientist (No. 30200115).

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Yuan, L., Renliang, W., Xi, W. et al. Effects of retinoic acid on the β-catenin/TCF pathway in culture porcine tracheobronchial epithelial cells. Current Medical Science 24, 421–423 (2004).

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