Skip to main content
SpringerLink
Log in

Applied study on magnetic nanometer beads in preparation of genechip samples

  • Published:
Current Medical Science Aims and scope Submit manuscript

Summary

A protocol for enrichment and adsorption of karyocyte from whole blood by using magnetic nanometer beads as solid-phase absorbents was presented. The PCR amplification could be accomplished by using the nanobeads with karyocyte as template directly and the PCR products were applied on an oligonucleotide array to do gene typing. The HLA-A PCR amplification system and a small HLA-A oligonucleotide microarray were applied as the platform and an experiment protocol of separating karyocyte from whole blood using the magnetic nanometer beads (Fe2O3) were set up. The experimental conditions were also discussed. It showed that pH level of PBS eluent, Taq enzyme quantity and fragment length of products could influent the amplification results, and the magnetic nano-beads could succeed in sample preparation in microarray to provide a promising way in automatic detection and lab-on-a-chip.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Rudi K, Kroken M, Dahlberg O Jet al. Rapid, universal method to isolate PCR-ready DNA using magnetic beads. Biotechniques, 1997, 22: 506

    Article  CAS  PubMed  Google Scholar 

  2. Deggerdal A, Larsen F. Rapid isolation of PCR-ready DNA from blood, bone marrow and cultured cells, based on paramagnetic beads. Biotechniques, 1997, 22: 554

    Article  CAS  PubMed  Google Scholar 

  3. Davis M J, Taylor J I, Sachsinger Net al. Isolation of plasmid DNA using magnetite as a solid-phase absorbent. Anal Biochem, 1998, 262: 92

    Article  Google Scholar 

  4. Gao X, Jakobsen I B, Serjeantson S W. Characterization of the HLA-A polymorphism by locus-specific polymerase chain reaction amplification and oligonucleotide hybridization. Hum Immunol, 1994, 41: 267

    Article  CAS  PubMed  Google Scholar 

  5. Petzl-Erler M L, McDevitt H O. Molecular analysis of the HLA DRB genes in two tribes of Brazilian Indians. Hum Immunol, 1994, 41: 180

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Chen Hui & Yang Yuzhen

  2. Department of Biological Science and Biotechnology, Tsinghua University, 100084, Beijing

    Gao Huafang

  3. Biochip Beijing National Engineering and Research Center, 100084, Beijing

    Gao Huafang & MA Xuemei

  4. Department of Chemical Engineering, Tsinghua University, 100084, Beijing

    Xie Xin

Authors
  1. Chen Hui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gao Huafang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xie Xin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. MA Xuemei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yang Yuzhen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

CHEN Hui, female, born in 1974, Ph. D candidate

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hui, C., Huafang, G., Xin, X. et al. Applied study on magnetic nanometer beads in preparation of genechip samples. Current Medical Science 24, 25–27 (2004). https://doi.org/10.1007/BF02830698

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02830698

Key words

Search

Navigation