Abstract
Hemagglutinin gene of Measles virus(Nepal strain) was amplified by RT-PCR technique, cloned and sequenced by the dideoxy-mediated chain termination method. The comparison to the standard strain (Edmonston strain) showed many important mutations. The homology of these two strains was 98.17%. Then H gene was cloned into expression vector pCD-SRα296 and introduced into COS-7 cells by electroporation method. The expression and function of cloned H gene was checked by hemadsorption assays.
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Supported by company of microbial diseases, Osaka university, Japan
Li Lingyun: born in sept. 1967. Ph. D, Graduate student
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Lingyun, L., Yipeng, Q. Measles virus (Nepal strain) hemagglutinin gene: Cloning, complete nucleotide sequence analysis and expression in COS cells. Wuhan Univ. J. Nat. Sci. 3, 377–382 (1998). https://doi.org/10.1007/BF02829998
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DOI: https://doi.org/10.1007/BF02829998