Abstract
An interspecies conservedPlasmodium asparagine rich antigen, designated as ARK26, was isolated by immunoscreeningP. falciparum genomic DNA expression library with mouse convalescent anti-P. yeolii serum. Partial DNA sequence analysis reveals that ARK26 contains clusters of asparagines and no randomly repeated amino acid sequence motifs are observed. A 65×103 GST fusion protein is expressed by recombinant plasmid PGEX-5X-1 (ARK26) inE. coli C strain ABLE-K. Computer programs predict that two asparagine rich regions are among the possible antigenic epitopes of p37 encoded by ARK26. Interestingly, the sequence of ARK26 displays significant similarity to yeast and several other species’ mitochondrial genes, and its possible function is discussed.
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Abbreviations
- GST:
-
glutathione-S-Transferase
- CSP:
-
circumsporozoite protein
- kb:
-
kilobase pairs
- IPTG:
-
isopropyl-β-D-thiogalactoside
- NCBI:
-
National Center of Biotechnology Information
- PHD:
-
prediction program of protein secondary structure
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
- IFAT:
-
Immunofluorescent Antibody Test
- ACRs:
-
Ancient Conserved Regions
- CARP:
-
clustered-asparagine-rich protein
- Asn:
-
asparagine
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Supported by a fellowship offered by International Center for Genetic Engineering & Biotechnology(ICGEB)
Ma Donghui: born in 1969, Graduate student
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Donghui, M., Xiaojie, L., Huiwen, M. et al. An interspecies conserved antigen ofPlasmodium falciparum containing clusters of asparagine residues. Wuhan Univ. J. Nat. Sci. 3, 369–376 (1998). https://doi.org/10.1007/BF02829997
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DOI: https://doi.org/10.1007/BF02829997