Abstract
Copper microelectrodes were used in microvoltammetry to detect histamine and several amino acid neurotransmitters. High sensitivity was obtained through the use of copper microelectrodes, and detection limits of 50 nmol/L and 90 nmol/L were obtained for histamine and histidine, respectively. Furthermore, histamine and several amino acids neurotransmitters were firstly separated and detected by capillary electrophoresis with amperometric detection on copper microelectrodes. Mass limits of 490 amol and 440 amol were achieved for histamine and histidines, respectively, by using this mothod.
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Ewing A G, Mesaros J M, Gavin P F. Electrochemical detection in microcolumn separations.Anal Chem, 1994,66(9): 527A-537A
Hu S, Pang D W, Li P B,et al. Electrochemical detection in capillary electrophoresis.J Instru Anal, 1997,16 (6): 70–75.
Gaitonde C D, Pathak P V. Capillary zone electrophoretic separation of chlorophenols in industrial waste water with on-column electrochemical detection.J Chromatogr, 1992,514(1/2): 389–393
O’Shea T J, Lunte S M, LaCourse W R. Detection of carbohydrates by capillary electrophoresis with pulsed amperometric detection.Anal Chem, 1993,65(7): 948–951
Lu W, Cassidy R M. Evaluation of ultramicroelectrodes for the detection of metal ions separated by capillary electrophoresis.Anal Chem, 1993,65,(13): 1649–1653
Ye J, Baldwin R P. Determination of amino acids and peptides by capillary electrophoresis and electrochemical detection at a copper electrode.Anal Chem, 1994,66 (17): 2669–2672.
Hu S, Hu Y G, Li P B,et al. On-column field-amplified capillary electrophoresis with amperometric detection.Chinese J Anal Chem, 1996,24(9): 1028–1031
Hu S, Wang Z L, Li P B,et al. Amperometric detection in capillary electrophoresis with etched joint.Anal Chem, 1997,69(2): 264–267
Luo P, Zhang F, Baldwin R P. Constant potential amperometric detection of underivatized amino acids and peptides at a copper electrode.Anal Chem, 1991,63 (17): 1702–1707
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Supported by the National Natural Science Foundation of China
Hu Shen: born in 1970, Ph. D.
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Shen, H., Weihua, H., Daiwen, P. et al. Amperometric detection of histamine and amino acid neurotransmitters in capillary electrophoresis with copper microelectrodes. Wuhan Univ. J. Nat. Sci. 3, 341–344 (1998). https://doi.org/10.1007/BF02829990
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DOI: https://doi.org/10.1007/BF02829990