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A potential pathological role of angiopoietins expression in glomeruli during progressive glomerulisclerosis related to podocyte injury

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Summary

A potential pathological role of angiopoietins (Ang) in glomeruli following podocyte injury-induced progressive glomerulosclerosis was explored. Eighty male Wistar rats were randomly allocated into sham operation group (Sham,n=25), Uninephrectomy group (UPHT,n=25) and Uninephrectomy+Daunorubicin group (DRB,n=30). In DRB group, daunorubicin (5 mg/kg) was injected via tail vein on the 7th and 14th day after uninephrectomy. At week 1, 2, 4, 6 and 8 respectively following establishment of the animal model, 5 rats in Sham group and UPHT group, and 6 in DRB group were taken respectively for determining 24-h urinary protein excretion rate (24hUPER), blood urea nitrogen (BUN) and serum creatinine (Scr). The sections of kidneys were examined by an electric microscope, PAS staining, immunohistochemical staining and in situ hybridization histochemistry. The results showed that 24hUPER, BUN and Scr in DRB group were more than those in Sham group and UPHT group at the same time points, and there was a trend towards an increase on level of GSI in DRB group from week 2 to week 8. Electric microscopy revealed that podocyte injury presented in DRB group. The expression of Angl mRNA and protein in glomeruli of DRB group was decreased, while the expression of Ang2 protein in glomeruli of DRB group increased, Meanwhile, the expression of Angl mRNA had a negative correlation with expression of Ang2 mRNA, and the expression of Angl protein had a positive correlation with the expression of Ang1 mRNA, and had a negative correlation with 24hUPER, BUN, Scr, glomerular sclerotic index (GSI), the expression of Ang2 protein and CoIV protein. The expression of Ang2 protein had a positive correlation with the expression of Ang2 mRNA, and had a positive correlation with 24hUPER, BUN, Scr, GSI, the expression of CoIV protein. It was concluded that podocyte injury might lead to an alteration in the expression of Ang1 and Ang2 within glomeruli. Ang2 may get rid of inhibition from Angl for downregulation of the Ang1 expression, which facilitate upregulation of the Ang2 expression in glomeruli to promote progressive glomerulosclerosis in the rats.

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References

  1. Angiopoietins: microvascular modulators with potential roles in glomerular pathophysiology. J. Nephrol, 2003, 16(2): 168–178

  2. Satchell, S C, Anderson K L, Mathieson P W. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties. J Am Soc Nephrol, 2004, 15(3):566–574.

    Article  PubMed  CAS  Google Scholar 

  3. Lu Y H, Deng A G, Li Net al. Changes in angiopoietin expression in glomeruli involved in glomerulosclerosis in rats with daunorubicin-induced nephrosis. Acta Pharmacol Sin (Chinese), 2006, 27(5):579–587.

    Article  CAS  Google Scholar 

  4. Beck H, Acker T, Wiessner Cet al. Expression of angiopoietin-1, angiopoietin-2, and tie receptors after middle cerebral artery occlusion in the rat. Am J Pathol, 2000, 157(5):1473–1483.

    PubMed  CAS  Google Scholar 

  5. Gamble J R, Drew J, Trezise Let al. Angiopoietin-1 is an antipermeability and anti-inflammatory agent in vitro and targets cell junctions. Circulation Res, 2000, 87(7): 603–607

    PubMed  CAS  Google Scholar 

  6. Ohtake T, Kimura M, Takemura Het al. Effects of dictary lipids on daunomycin-induced nephropathy in mice: comparison between cod liver oil and soybean oil. Lipids, 2002, 37(4):359–366.

    Article  PubMed  CAS  Google Scholar 

  7. Haggstrom Rudolfsson S, Johansson A, Franck Lissbrant I,et al. Localized expression of angiopoietin 1 and 2 may explain unique characteristics of the rat testicular microvasculature. Biol Reprod, 2003, 69(4):1231–1237.

    Article  PubMed  Google Scholar 

  8. Singh A K, Gudehithlu K P, Pegoraro A Aet al. Vascular factors altered in glucose-treated mesangial cells and diabetic glomeruli. Changes in vascular factors impair endothelial cell growth and matrix. Lab Invest, 2004, 84(5):597–606

    Article  PubMed  CAS  Google Scholar 

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Author notes

  1. Lu Yuanhang

    Present address: Department of Nephrology, Zhongshan Hospital of Hubei Province, 430030, Wuhan, China

Authors and Affiliations

  1. Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China

    Lu Yuanhang, Deng Anguo, Yang Xiao, Liu Jianshe & Zhu Zhonghua

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  1. Lu Yuanhang
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  2. Deng Anguo
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  3. Yang Xiao
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  4. Liu Jianshe
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  5. Zhu Zhonghua
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Additional information

LU Yuanhang, male, born in 1964, M. D., Ph. D., Associate Professor.

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Yuanhang, L., Anguo, D., Xiao, Y. et al. A potential pathological role of angiopoietins expression in glomeruli during progressive glomerulisclerosis related to podocyte injury. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 26, 294–297 (2006). https://doi.org/10.1007/BF02829555

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  • DOI: https://doi.org/10.1007/BF02829555

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