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Transforming growth factor-β2 gene cloning and protein expression in human trabecular meshwork cells

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Summary

Whether cultured human trabecular meshwork cells express transforming growth factor-β2 (TGF-β2) messenger RNA (mRNA) and protein was investigated. Total RNA of 106 cultured human trabecular meshwork cells was extracted with TRIZOL reagent, reverse transcriptase-polymerase chain reaction (RT-PCR) were used for detection of TGF-β2 messenger RNA, and the PCR product was verified by sequencing. Immunohistochemical staining was used to detect TGF-β2 protein. The results showed that a single RT-PCR amplified product was obtained, and the sequence was homologous to the known sequence. TGF-β2 immunostaining was positive. It was concluded that trabecular meshwork cells could produce TGF-β2 and contribute to the presence of TGF-β2 in trabecular meshwork microenvironment as well as aqueous humor. Trabecular meshwork cells were affected by TGF-β2 not only through paracrine, but also autocrine action. Whether abnormal changes in TGF-β2 production contribute to the pathogenesis of primary open-angle glaucoma is worth further investigation.

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Authors and Affiliations

  1. Department of Ophthalmology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan

    Cao Yang & Wei Houren

  2. Department of Emergency, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan

    Da Banghong

  3. Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Li Zhongyu

Authors
  1. Cao Yang
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  2. Wei Houren
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  3. Da Banghong
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  4. Li Zhongyu
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Additional information

CAO Yang, male, born in 1972, M. D., Ph. D., Doctor in Charge

This project was supported by the National Natural Science Foundation of China (Serial No. 38970758).

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Yang, C., Houren, W., Banghong, D. et al. Transforming growth factor-β2 gene cloning and protein expression in human trabecular meshwork cells. Current Medical Science 23, 85–87 (2003). https://doi.org/10.1007/BF02829473

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  • DOI: https://doi.org/10.1007/BF02829473

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