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Determination of progesterone receptor by chemiluminescent enzyme immunoassay

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Summary

A new method of chemiluminescent enzyme immunoassay (CLEIA) was developed and the standard curve and regression equation for determination of progesterone receptor (PR) made. The luminosity of tissue samples was tested and PR level was calculated by the regression equation. Correlation analysis revealed that there was a linear relationship between different concentrations of the standard PR samples and the corresponding values of luminosity: Y=3748+463.77X, γ=0.9958. The values of the luminosity in 38 cases of tumor tissues were determined with the highest being 267.32 fmol/mg, the lowest 3.69 fmol/mg and the mean 78.53 fmol/mg. The new method of CLEIA was a stable, creditable, specific and sensitive assay for determination of PR.

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Authors and Affiliations

  1. Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Yin Tiejun

  2. Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Gu Meijiao, Zheng Wen, Hu Wei & Lu Yunping

  3. School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Zhou Yikai

Authors
  1. Yin Tiejun
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  2. Gu Meijiao
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  3. Zhou Yikai
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  4. Zheng Wen
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  5. Hu Wei
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  6. Lu Yunping
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Additional information

YIN Tinejun, male, born in 1965, Doctor in Charge

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Tiejun, Y., Meijiao, G., Yikai, Z. et al. Determination of progesterone receptor by chemiluminescent enzyme immunoassay. Current Medical Science 23, 60–61 (2003). https://doi.org/10.1007/BF02829465

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  • DOI: https://doi.org/10.1007/BF02829465

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