Summary
The effect of thyrosine kinase, calmodulin and voltage-dependent Ca2+ channel on the proliferation of hepatoma cells induced by EGF was studied. Hepatoma cell line SMMC7721 was cultured in RPMI1640 serum-free medium. DNA synthesis rate of hepatoma cells was measured by H-TdR incorporation. 10−9 mol/L EGF could significantly stimulate the proliferation of hepatoma cells (P<0.05), and this effect might be significantly inhibited by tyrosine kinase inhibitor (P<0.001). Calmodulin inhibitor W-7 had no effect on the basic phase of cultured hepatoma cells (P>0.05), but it had very significantly inhibitory effect on the proliferation of hepatoma cells induced by EGF (P<0.001). Voltage-dependent Ca2+ channel inhibitor Varapamil had no inhibition on the proliferation of hepatoma cells induced by EGF (P>0.05). It had no effect on the basic phase of cultured hepatoma cells, (P>0.05). It is suggested that tyrosine kinase and Ca2+-calmodulin-dependent pathway may play a critical role on the proliferation of heptoma cells induced by EGF and voltage-dependent Ca2+ channel is independent of the effect of EGF.
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Francavilla A, Barone M, Azzarone Aet al. Cell regeneration in the pathobiology of liver carcinomas. Gas-troenterology, 1991, 23: 589
Hung W C, Chuang L Y, Tsai J Het al. Effects of epidermal growth factor on growth control and signal transduction pathways in different human hepatoma cell lines. Biochem Mol Biol Int, 1993, 30(2): 319
Gilligan A, Prentki M, Glennon M Cet al. Epidermal growth factor-induced increase in inositol trisphosphates, inositol tetrakisphosphates, and cytosolic Ca2+ in a human hepatocellular carcinoma-derived cell line. FEBS Lett, 1988, 233: 41
Nakajima M, Murata T, Yamaguchi M. Expression of calcium-binding protein regucalcin mRNA in the cloned rat hepatoma cell (H4-I-E) is stimulated through Ca2+ signal factor: involvement of protein kinase C. Mol Cell Biochem, 1999, 198(1–2): 101
Tamura N, Tai Y, Sugimoto Ket al. Enhanced expression and activation of Ca2+/calmodulin-dependent protein kinase N in hepatocellular carcinoma. Cancer, 2000, 89 (9): 1910
Grandis J R, Drenning S D, Chakraborty Aet al. Requirement of Stat 3 but not Stat1 activation for epidermal growth factor receptor-mediated cell growth in Vitro. J Clin Invest, 1998, 102(7): 1385.
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WU Binwen, male, born in 1968, M. D., Ph.D.
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Binwen, W., Jialong, W., Shunyu, Y. et al. Effect of calmodulin and voltage-dependent Ca2+ channel on the proliferation of heptoma cells induced by epidermal growth factor. Current Medical Science 23, 26–28 (2003). https://doi.org/10.1007/BF02829454
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DOI: https://doi.org/10.1007/BF02829454