Summary
To investigate the expression of NOS III mRNA and protein in cultured porcine cerebral arterial endothelial cells (CAEC) during hypoxia and reoxygenation and the effects of L-Tetrahydropalmatine (L-THP) on the gene expression of NOS III in CAEC during hypoxia and reoxygenation. The cultured CAEC were divided into 5 groups; control, hypoxia, hypoxia+reoxygenation, hypoxia +L-THP and reoxygenation +L-THP groups. NOS III mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR). Immunocytochemistry was used to detect the level of NOS III protein. The expression of NOS III mRNA and protein were increased when CAEC were exposed to hypoxia for 1 h, and significantly decreased during reoxygenation 2, 6 and 12 h after 1-h of hypoxia. L-THP from 10−8 mol/L to 10−3 mol/L could inhibit the up-regulation of NOS III gene expression during hypoxia and down-regulation of NOS III gene expression during reoxygenation.
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References
Yang G T, Wang P H, Tang Yet al. Effects of L-Tetrahydroplmatine on energy metabolism, endothelin-1 and NO during acute cerebral ischemia-reperfusion of rats. J Tongji Univ, 1999, 19(4):285
Woodman C R, Muller J M, Laughlin M Het al. Induction of nitric oxide synthase mRNA in coronary resistance arteries isolated from exercise-trained pigs. Am J Physiol, 1997, 273:H2575
Karri K. Bradley, William J. Hatton, Helens Masovet al. Kir3. 1/3. 2 encodes an Ikach-like Current in gastrointestinal myocytes. Am J Physiol, 2000, 278(2): G289
Beckman J S. Apparent hychoxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci USA, 1991, 87:1620
Urs A, Arnet, Auchey Mcmillanet al. Regulation of endothelial nitric-oxide synthase during hypoxia. J Biol Chem, 1996, 271 (25):15069
Moncada S, Palmer R M J, Higgs E A. The Discovery of Nitric Oxide Endogenous Nitrovasodilator. Hypertension, 1998, 12:365
Knapp L T, Kanterewicz B I, Hayes E. Peroxynitrite-induced tyrosine nitration and inhibation of protein kinase C. Bioch Bioph Res Comm, 2001, 286:764
Li H G, Oehrlein S A, Wullerth Tet al. Activation of protein kinase Cα and/or ζ enhances transcription of the human endothelial nitric oxide synthase gene. Mol Pharmacol, 1998, 53:630
Stuart I M D, Ray Het al. Possible role for oxygen free radicals in the regulation of renal nitric oxide synthase and blood flow. Am J Surg, 1995, 169:604
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YANG Guangtian, male, born in 1952, Professor
This project was supported by a grant from the key Project of Science and Technology Brainstorm Program of Hubei Province (Serial No. 2001AA307B06).
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Guangtian, Y., Zhenju, S., Deqin, L. et al. Effects of L-tetrahydropalmatine on NOS III gene expression in hypoxia and cultured porcine cerebral arterial endothelial cells during reoxygenation. Current Medical Science 23, 19–22 (2003). https://doi.org/10.1007/BF02829452
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DOI: https://doi.org/10.1007/BF02829452