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Development of multiplex polymerase chain reaction for the detection and differentiation of enteric adenoviruses in stool samples

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Summary

To find a technique of detecting and differentiating enteric adenoviruses (EAds) in clinical samples, a novel PCR approach was developed. EAds were able to be detected by use of a pair of subgroup F general primers (P1 and P2), and they were also be able to be differentiated from each other in the presence of another adenovirus type 40 (Ad40) specific primer (P3) in the same tube. Our results showed that there was one band for Ad41 and two bands for Ad40, respectively, on running-gel after PCR performance. PCR was performed on 40 specimens in parallel directly with dot-hybridization assay on the same diluted stool samples. 20 of 40 specimens were positive by hybridization (of them 12 were Ad41 and 8 were Ad40), whereas 26 were positive by PCR performance on the same samples with Ad41 18 and Ad40 8 positive as well. Our study indicated that this novel method could be used in clinical laboratory or in epidemic investigation for Eads

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References

  1. Uhnoo I, Wadell G, Svensson Let al. Importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. J clin Microbiol, 1984, 20: 365

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Allard A, Girones R, Juto Pet al. Polymerase chain reaction for detection of adenoviruses in stool samples. J Clin Microbiol, 1990, 28: 2659

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Allard A, Albinsson B, Wadell G. Detection of adenoviruses in stools from healthy persons and patients with diarrhea by two-step polymerase chain reaction. J Med Virol, 1992, 37: 149

    Article  CAS  PubMed  Google Scholar 

  4. Elnifro E M, Cooper R J, Klapper P Eet al. PCR and restriction endonuclease analysis for rapid identification of human adenovirus subgenera. J Clin Microbiol, 2000, 38: 2055

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Pathogenic Biology, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Zhang Weidong, Zhu Yuze, Wang Bei, Qu Hong, Cao Xueqin, Huang Qinghua & Chen Xiuzhu

  2. Institute of Family Planning Research, Tongji Medical College Huazhong University of Science and Technology, 430030, Wuhan

    Yang Hong

Authors
  1. Zhang Weidong
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  2. Yang Hong
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  3. Zhu Yuze
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  4. Wang Bei
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  5. Qu Hong
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  6. Cao Xueqin
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  7. Huang Qinghua
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  8. Chen Xiuzhu
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Additional information

ZHANG Weidong, male, born in 1964, Associate Professor

This project was supported by a grant from National Sciences Foundation of China (Serial No. 39370038) and part of a grant from Hubei Science and Technology Committee (No. 89JJ13-18).

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Weidong, Z., Hong, Y., Yuze, Z. et al. Development of multiplex polymerase chain reaction for the detection and differentiation of enteric adenoviruses in stool samples. Current Medical Science 23, 1–3 (2003). https://doi.org/10.1007/BF02829446

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  • DOI: https://doi.org/10.1007/BF02829446

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