Summary
The constitutive expression of nuclear-factor-κB (NF-κB) in human pigment epithelial (hRPE) cells cultivatedin vitro and the possible changes when incubated with PDTC and IL-1β were investigated. The synchronized hRPE cellsin vitro were divided into two groups. In non-PDTC group, hRPE cells were exposed respectively to IL-1βand NS (for detecting the constitutive expressions of NF-κB in hRPE cells); In PDTC group, PDTC-pretreated hRPE cells were exposed respectively to IL-1βand NS (for detecting the constitutive expression of NF-κB in PDTC-pretreated hRPE cells). The expression of NF-κB in hRPE cells in two groups was detected by immunofluorescence stain and flow cytometry. The results showed that the constitutive expression of NF-κB in hRPE cellsin vitro was 8.05%, and increased to 30.26% by IL-1β. After PDTC pretreatment, the constitutive expression of NF-κB in hRPE cells was decreased to 3.74%, and 3.66% by IL-1βrespectively. It was concluded that the expressions of NF-κB in hRPE cells could be increased significantly by IL-1βand depressed effectively by PDTC. Also, PDTC could significantly inhibit the activation of NF-κB induced by IL-1β.
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HU Jun, male, born in 1970, Doctor in Charge
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Jun, H., Guigang, L., Jianbin, C. et al. Expression of NF-κB in hRPE cells stimulated by PDTC and IL-1β. Current Medical Science 25, 362–364 (2005). https://doi.org/10.1007/BF02828169
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DOI: https://doi.org/10.1007/BF02828169