Summary
The expression of CD44v6 and its relationship with the development, progress, invasion and metastasis of laryngeal carcinoma was investigated. The expression and content of CD44v6 mRNA in tissuess were detected by both RT-PCR and FCM which were respectively extracted from normal laryngeal mucosa, leukoplakia of larynx, laryngeal papilloma, polyp of vocal cord, tissues of laryngeal carcinoma, metastatic and nonmetastatic lymph nodes of neck, and tissues close to carcinoma. the outcome of RT-PCR indicated that the expression rate of CD44v6 mRNA involved in tissues of laryngeal carcinoma and metastatic lymph nodes of neck was the highest (90%–100%) compared with that of leukoplakia of larynx, laryngeal papilloma, tissues close to carcinoma by 0.5 cm (55.56%–60.00%) and that of normal laryngeal mucosa, polyp of vocal cord, nonmetastatic lymph nodes and tissues close to carcinoma by 1.0 cm was the lowest (13.33%–20%). The result from FCM was highly consistent with that from RT-PCR. It was suggested that CD44v6 was closely related with the development, progress, invasion and metastasis of laryngeal carcinoma. The outcome from the tissues close to carcinoma by different distance could do help to the determination of incisal edge in surgery abstractly.
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References
Van Hal N L, Van Dongen G A, Stigter-van Walsum Met al. Characterization of CD44v6 isoforms in head-and neck squamous cell carcinoma. Int J Cancer, 1999, 82(6): 837
Stickeler E, Mobus V J, Kieback D Get al. Intron 9 retention in gene transcripts suggests involvement of CD44 in the tumorigenesis of ovarian cancer. Anticancer Res, 1997, 17(6D): 4395
Sun X M, Yin G P. Establishment of flow cytometry about cell adhesion molecule in tissues of solid tumor mass. Clin J Med Lab Sci (Chinese), 1997, 20: 9
Wang J, Wang M, Zhang Yet al. The detection of CD44v6 and clinical significance in tissues of carcinoma of endometrium. Zhongguo Shi Yong Fu Ke Yu Chan Ke Za Zhi (Chinese), 2003, 19: 165
Ishida T. Immunohistochemical expression of the CD44 variant 6 in colorectal adenocarcinoma. Surg Today, 2000, 30(1): 28
Sun B C, Zhao S W, He Jet al. Proliferation nucleus antigen: CD44 protein expression and clinical significance in tissues of laryngeal carcinoma. Di Er Jun Yi Da Xue Xue Bao (Chinese), 2003, 24: 77
Kaufmann M, Heider K H, Sinn H Pet al. CD44 variant exon epitopes in primary breast cancer and length of survival. Lancet, 1995, 345(8950): 615
Guler G, Sarac S, Uner Aet al. prognostic value of CD44 variant 6 in laryngeal epidermoid carcinomas. Arch Otolaryngol Head Neck Surg, 2002, 128(4): 393
Maula S M, Luukkaa M, Grenman Ret al. Intratumoral lymphatics are essential for the metastatic spread and prognosis in squamous cell carcinomas of the head and neck region. Cancer Res, 2003, 63(8): 1920
Sikorska B, Danilewicz M, Wagrowska-Danilewicz M. Prognostic significance of CD44v6 and nm23 protein immunoexpression in laryngeal squamous cell carcinoma. Pol J Pathol, 2002, 53(1): 17
Zhou L, Ding P, Wang W. The relation between local relapse and incisal edge in surgery of laryngeal carcinoma. J Clin Otorhinolaryngol (Chinese). 2004, 18: 339
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LIU Banghua, male, born in 1962, Associate Professor
This project was supported by a grant from Hubei Provincial Natural Sciences Foundation (98J118).
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Banghua, L., Weijia, K., Shusheng, G. et al. Relationship between the expression of CD44v6 and development, progress, invasion and metastasis of laryngeal carcinoma. Current Medical Science 25, 351–353 (2005). https://doi.org/10.1007/BF02828165
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DOI: https://doi.org/10.1007/BF02828165