Summary
Some biological characteristics of human bone marrow mesenchymal stem cells (MSCs) culturedin vitro were observed. hMSCs were isolated from bone marrow and purified by density gradient centrifugation method, and then culturedin vitro. The proliferation and growth characteristics of hMSCs were observed in primary and passage culture. MSCs of passage 3 were examined for the purify by positive rate of CD29 and CD44 through flow cytometry. Human bone marrow MSCs showed active proliferation capacityin vitro. The purify of MSCs separated by our method was higher than 90%. It was concluded that hMSCs have been successfully cultured and expanded effectively. It provided a foundation for further investigation and application of MSCs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Makino S, Fukuda K, Miyoshi Set al. Cardiomyocytes can be generated from marrow stromal cellsin vitro. J Clin Invest, 1999, 103:697
Pittenger M F, Mackay A M, Beck S Cet al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999, 284:143
Haynesworth S E, Goshima J, Goldberg V Met al. Characterization of cells with osteogenics potential from human marrow. Bone, 1992, 13(1):81
Pereira R F, Halford K M, O’Hara M Det al. Cultured adherent cells from marrow can serve as longlasting precursor cells for bone, cartilage and lung in irradiated mice. Proc Natl Acad Sci USA, 1995, 92(11): 4857
Prockop D J. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science, 1997, 276(5):71
Bruder S P, Kurth A A, Shea Met al. Bone regeneration by implanation of purified, culture-expanded human mesenchymal stem cells. J Orthop Res, 1998, 16 (2):1551
Ai G P, Su Y P, Yan G Het al. Isolation and culture of bone marrow mesenchymal stem cells. Acta Academiae Medicinae Militaris Tertiae, 2001, 23(5):553
Caplan A I, Bruder S P. Mesenchymal stem cells: building blocks for molecular medicine in the 21st century. Trends Mol Med, 2001, 7:259
Ohgushi H, Caplan A I. Stem cell technology and biocramics: from cell to gene engineering. J Biomed Mater Res, 1999, 48(6):913
Stan Gronthos, Andrew C W, shelley J Het al. Molecular and cellular characteristic of highly purified stromal stem cells derived from human bone marrow. J Cell Science, 2003, 116:1827
Deans R J, Moseley A B. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol, 2000, 28:875
Author information
Authors and Affiliations
Additional information
FA Xian’en, male, born in 1962, Professor
Rights and permissions
About this article
Cite this article
Xian’en, F., Lixia, W., Jianfeng, H. et al. Biological characteristics of human bone marrow mesenchymal stem cell culturedin vitro. Current Medical Science 25, 307–309 (2005). https://doi.org/10.1007/BF02828151
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02828151