Summary
The effect of electroacupuncture (EA) on TRPM7 mRNA expression of focal cerebral ischemia in rats and further the role of EA in the relationship between TRPM7 and trkA pathway was investigated. Thirty SD rats were randomly divided into 5 groups: normal group, ischemia/reperfusion group, EA treated group (ischemic rats with EA treatment), TE infusion group (ischemic rats with EA treatment and TE buffer infusion), AS-ODN group (ischemic rats with EA treatment and antisense trkA oligonucleotide infusion). The stroke animal model was established by the modified method of middle cerebral artery occlusion. Antisense trkA oligonucleotide that blocked NGFs effects was injected into cerebroventricle before EA. The TRPM7 mRNA was detected by RT-PCR method. The results showed that there were low TRPM7 mRNA levels in cortex and hippocampus in normal group. Compared with normal group, TRPM7 mRNA expression was increased significantly in ischemia/reperfusion group (P<0.05). A significant reduction in the expression of TRPM7 mRNA was found in EA treated group in contrast to ischemia/reperfusion group (P<0.05). The expression of TRPM7 mRNA in AS-ODN group was remarkably increased compared with EA treated group and TE infusion group (P<0.05). The results indicated that TRPM7 channels in the ischemic cortex and hippocampus in rats might play a key role in ischemic brain injury. EA could reverse the overexpression of TRPM7 in cerebral ischemia/reperfusion rats. And the inhibitory effect of EA on TRPM7 channels might be through trkA pathway.
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References
Aarts M, Iihara K, Wei W Let al. A key role for TRPM7 channels in anoxic neuronal death. Cell, 2003, 115 (7): 863
Shi J. A study on the effect and mechanism of acupuneture suppression of neuronal apoptosis following cerebral ischemia. Progress in Physiological Sciences, 1999, 30(4): 326
Shi J, Liu X C, Zhang Jet al. The influence of acupuncture on the NGF receptor—TrkA of focal cerebral ischemia in rats. Chinese Journal of Histochemistry and Cytochemistry, 1999, 8(2):132
Longa E Z, Weinstein P R, Carlson Set al. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke, 1989, 20(1): 84
Woolf N J, Milov A M, Schweitzer E Set al. Elevation of recve grouth factor and antisense knockdown of TrkA receptor during contextual memory consolidation. J Neurosci, 2001, 21(3): 1047
Jiang X, Newell E W, Schlichter L Cet al. Regulation of a TRPM7-like current in rat brain microglia. J Biol Chem, 2003, 278(44): 42 867
Shi J, Liu X C, Zhang Jet al. The influence of acupuncture on the NMDAR1 mRNA after focal cerebral ischemia following reperfusion in brain of rats. Chinese Journal of Histochemistry and Cytochemistry, 2000, 9 (4): 371
Heurteux C, Lauritzen I, Widmann Cet al. Glutamate-induced overexpression of NMDA receptor messenger RNAs and protein triggered by activation of AMPA/kainate receptors in rat hippocampus following forebrain ischemia. Brain Res, 1994, 659 (1–2): 67
Holtzman D M, Sheldon R A, Jaffe Wet al. Nerve growth factor protects the neonatal brain against hypoxic-ischemic injury. Ann Neurol, 1996, 39(1): 114
Kaplan D R, Miller F D. Neurotrophin signal transduction in the nervous system. Curr Opin Neurobiol, 2000, 10 (3): 381
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ZHAO Li, female, born in 1978, Postgraduate Student
This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30472234).
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Li, Z., Jing, S., Ning, S. et al. Effect of electroacupuncture on TRPM7 mRNA Expression after cerebral ischemia/reperfusion in rats via TrkA pathway. Current Medical Science 25, 247–250 (2005). https://doi.org/10.1007/BF02828132
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DOI: https://doi.org/10.1007/BF02828132