A primary physical map of rice chromosome 12 was constructed using marker-based chromosome landing and chromosome walking. A BAC library from IR64 was screened using 84 RFLP markers, 4 STS markers and 6 microsatellite markers on chromosome 12 by colony hybridization and polymerase chain reaction (PCR) amplification. A total of 59 contigs consisting of 419 BAC clones including 5 single-clones were physically aligned on rice chromosome 12 with the largest BAC contig covering 855 kb. The whole physical map had a size of ∼16 Mb and covered about 52% of rice chromosome 12. This physical map will be certainly helpful for map-based gene cloning of agronomically and biological important genes and understanding the genome structure of the chromosome.
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Foundation item: Supported by Rockefeller Foundation
Biography: FU Bin-Ying (1965-), male, Ph. D. candidate, Reseach direction: plant molecular genetics.
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Bin-Ying, F., Ying-Guo, Z. & Zhi-Kang, L. Construction of the primary physical map of rice chromosome 12. Wuhan Univ. J. Nat. Sci. 5, 238–244 (2000). https://doi.org/10.1007/BF02827938
- chromosome landing
- chromosome walking
- BAC contigs
- physical map
- Q 343. 2