Abstract
The fluorescence intensity of the europium (III)-tetracycline (TC) complex can be greatly increased by TOPO, TBP, TTA and surfactans, among which TOPO showed the greatest enhancement. The formation of ternary complex was proposed to be responsible for the fluorescence enhancement. The Eu (III)-TOPO-TC system can be applied to the determination of trace amounts of tetracycline in urinous sample; the maximum fluorescence intensity was obtained in the pH range of 8.2∼9.5 with excitation and emission wave-lengths at 396 nm and 616 nm, respectively. The linear range for tetracycline is 6.0×10−9−5.0×10−7 mol·L−1. The detection limit is 2.0×10−9 mol·L−1(SNR=3). Experimental conditions and the mechanism of the enhancement effect are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Zhang K J, Lu Z L.Internal Medicine Lessons. Beijing: People's Health Press, 1982
Kelly R G, Peets L M, Hoyt K D. A fluorometric method of analysis for tetracycline.Anal Biochem, 1969,28: 222–229
Hayes J E, J R, Dubuy H G. A simple method for quantitative estimation of tetracycline antibiotics.Anal Biochem, 1964,7: 322–327
Hirschy L M, Dose E V, Winefordner J D. Lanthanidesensitized luminescence for the detection of tetracyclines.Anal Chim Acta, 1983,147: 311–316
Huang Hanguo, Hiraki K, Nishikawa Y. The fluorescence properties of europium and samarium β-Diketonates and their use in fluorometry.Journal of the Chemical Society of Japan, 1981,1: 66–73
Zhu Guiyun, Yang Jinghe, Si Zhikun. Study on the mechanism of the columinescence effect of rare earths.Journal of the Chinese Rare Earth Society, 1989,7: 73–78
Bhatt V K, Jee R D. Micro-ionization acidity constants for tetracyclines from fluorescence measurements.Anal Chim Acta, 1985,167: 233–24
Author information
Authors and Affiliations
Additional information
Supported by National Natural Science Foundation of China
Huang Chunbao: born in 1958, Associate professor
Rights and permissions
About this article
Cite this article
Chunbao, H., Yunxiang, C. & Wenbao, C. Fluorescence determination of tetracycline by Eu (III)/TOPO/TC system. Wuhan Univ. J. Nat. Sci. 4, 95–98 (1999). https://doi.org/10.1007/BF02827633
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02827633