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Studies on reduced extent method for inhibited thermokinetics of enzyme-catalyzed reaction

Inhibition of the laccase-catalyzed oxidation ofo-dihydroxybenzene bym-dihydroxybenzene

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Wuhan University Journal of Natural Sciences

Abstract

The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced, and then the criteria for distinguishing inhibition type was given and the methods for calculating kinetic parameters,K M,K i andv m were suggested. This theory was applied to inverstigate the inhibited thermokinetics of laccase-catalyzed oxidation ofo-dihydroxybenzene bym-dihydroxybenzene. The experimental results show the inhibition belongs to reversible competitive type,K M=6.224×10−3 mol·L−1,K i=2.363×10−2 mol·L−1.

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Authors and Affiliations

  1. Department of Chemistry, Wuhan University, 430072, Wuhan, China

    Xiong Ya, Wu Dingquan & Qu Songsheng

  2. Department of Environmental Science, Wuhan University, 430072, Wuhan, China

    Du Yumin

Authors
  1. Xiong Ya
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  2. Wu Dingquan
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  3. Qu Songsheng
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  4. Du Yumin
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Additional information

Xiong Ya: born Sep. 1961, Ph. D. graduate student. Curent research interest is in biothermochemistry research

Supported by the National Natural Science Foundation of China

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Ya, X., Dingquan, W., Songsheng, Q. et al. Studies on reduced extent method for inhibited thermokinetics of enzyme-catalyzed reaction. Wuhan Univ. J. of Nat. Sci. 1, 98–100 (1996). https://doi.org/10.1007/BF02827590

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  • DOI: https://doi.org/10.1007/BF02827590

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