Abstract
We transfected Sf9 cells with an expressing vector p35IE1Neo containing antiapoptotic p35 gene and neomycin-resistant gene (as a selection marker). By G418 screening, we got transformed cells that appeared resistant to G418 and picked one clone named Sf9-35. By hybridization in situ, it was found that p35 gene had integrated into the chromosome of Sf9-35 cells; By using actinomycin D treatment and cellular DNA electrophoresis, Sf9-35 cells were found to resist apoptosis induced by infection of vAcΔp35 deleting p35 gene and actinomycin D treatment; And it was also found that apoptosis induced by viral infection and actinomycin D treatment can only be delayed, but can not be stopped in Sf9-35.
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Supported by the National Natural Science Fundation of China
Li Xiaofeng: born in 1965, Lecturer
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Xiaofeng, L., Yipeng, Q., Hong, L. et al. Inhibiting mechanism of baculovirus p35 gene to apoptosis. Wuhan Univ. J. Nat. Sci. 3, 243–246 (1998). https://doi.org/10.1007/BF02827562
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DOI: https://doi.org/10.1007/BF02827562