Abstract
A novel fluorimetric method for determination of laccase activity in organic solvents is proposed, based on the oxidation ofo-phenylenediamine (1,2-diaminobenzene, OPDA) catalyzed by laccase yielding 2,3-diaminophenazine. The optimal conditions for laccase in organic media areT=55°C, pH=6.5, 1.0×10−2mol/L OPDA, 1.25 mL ethanol, 1.25 mL 1,4-dioxane and 1.25 mL acetone. The linear range of the method proposed in ethanol, 1,4-dioxane and acetone media were 0.44–19.33, 0.11–20.85, 0.38–21.05 U with the detection limit of 0.088, 0.022, 0.076 U, respectively. The proposed method has been applied to the analysis of laccase activity of real samples with more accurate and sensitive than that of the previous method reported.
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References
Dixon M, Webb E C.Enzymes, 3rd ed, New York: Academic Press, 1979, 220
Gigi O, Marbach I, Mayer A M. Properties of gallic acid-induced extracellular laccase ofbotrytis cinerea.Phytochemistry, 1981,20:1211–1213
Christine S E. Lactase activity in lignin degradation bycoriolus versicolor in vivo and in vitro Studies.FEMS Microbiol Lett, 1985,27, 339–343.
Badiani M, Fellci M, Luna,et al. Laccase assay by means of high-performance liquid chromatography.Anal Biochem, 1983,133:275–276
Shin T, Murao S, Matsumura E. A chromogenic oxidative coupling reaction of laccase and angiotensin I converting enzyme assay.Anal Biochem, 1987,166:380–388
Kurtz M B, Champe S P. Purification and characterization of the conidial laccase ofAspergillus Nidulans.J Bacteriol, 1982,151:1338–1345
Huang Houpng, Cai Ruxiu, Du Yumin,et al. Micelle enhanced spectrofluorimetric assay for laccase activity by a flow-injection stopped-flow technique.Anal Chim Acta, 1995,318:63–69
Houping Huang, Cai Ruxiu, Du Yumin,et al. The automatic kinetic spectrophotometric assay for laccase activity usingo-phenylenediamine.L R A, 1996,8:231–225
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Supported by the National Natural Science Foundation of China and the Special Founds of State Education Committee for Doctorate Research
Huang Zuyun: born in 1963, Ph.D
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Zuyun, H., Zhihong, L., Ruxiu, C. et al. Organic solvents enhanced spectrofluorimetric method for determination of laccase activity. Wuhan Univ. J. Nat. Sci. 3, 123–125 (1998). https://doi.org/10.1007/BF02827531
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DOI: https://doi.org/10.1007/BF02827531