Abstract
We have used the polymerase chain reaction to analyze variation in the size of 5S ribosomal gene spacer sequence. Eighty accessions, including 65 cultivated rice and 15 wild rice, were analyzed. Among them seven size classes of 5S DNA spacer were observed. Classification asindica orjaponica on the basis of 5S DNA spacer patterns generally agrees with classification based on morphological studies, indicating that the length polymorphism of 5S DNA spacer could be used as a molecular marker for taxonomic and phylogenetic analysis.
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Scols G J, Gill B S, Xin Z Y,et al. Frequent duplication and deletion events in the 5S RNA genes and the associated spacer region in the Trticease.Pl Syst Evol, 1988,160:105–112.
Doyle J J, Brown A H D. 5S ribosomal gene variation in the Glycine tomentalla polyploid complex (leguminosae).Syst Bot, 1989,14:398–407.
Dvorak J, Zhang H B, Kota R S,et al. Origination and evolution of the 5S ribosomal RNA gene family in wheat and related species.Genome, 1989,32:1003–1016.
Reddy P, Apples R. A second locus for the 5S multigene family in secale L: Sequence divergence in two lineages of the family.Genome, 1989,32:456–467
Cox A V, Bennet M D, Dyer T A. Use of the polymerase chain reaction to detect spacer size heterogenecity in plant 5S rRNA gene clusters and to locate such clusters in wheat.Theor Appl Genet, 1992,83:684–690
Moran G F, Smith D, Bell J C,et al. The 5S rRNA genes in pinus ratiata and the the spacer region as a probe for relationships between pinus species.Pl Syst Evol species Pl Syst Evol, 1992,183:209–221
Baum B R, Johnson D A. The 5S rRNA gene units in ancestral two-rowed barley.Genome, 1996,39:140–149.
Klassen G R. 5S rRNA genes spacers as species-specific probes for eight species of pythum.Phytopathology, 1996,86:581–587.
Hu J, Quiros C F. Identification of broccoli and cauliflower cultivars species of with RAPD markers.Plant Cell Report, 1991,10:505–511
Bassam B J, Caetano-Anoiles G, Gresshoff P M. Fast and sensitive silver staining of DNA in polyacrylamide gel.Anal Biochem, 1991,196:80–83
Mclntyre C L, Winberg B, Houchins K,et al. Relationships between oryza species (poacese) based on 5S DNA sequences.Pl Syst Evol, 1992,183:249–264
Yi Q M, Liu G P. Spacer length variation in rice 5S rRNA genes reveated by polymerase chain reaction.Wuhan University Journal of Natural Sciences, 1997,2 (1):124–126
Cheng K S, Wang X K, Zhou J W,et al. A revised classification of Asian cultivated rice.Chese Acta Agron Sin, 1984,42, 271–280
Glaszmann J C. Isozymes and classification of Asian rice.Theo Appl Genet, 1987,74: 21–30
Second G. Origion of the genic diversity of cultivated rice (Oryza sppl.): Study of the polymorphism scored at 40 isozyme loci.Jan J Genet, 1982,57:25–57
Kanazin V, Ananiev E, Blake T. The genetics of 5S rRNA encoding multigene families in barley.Genome, 1993,36:1023–1028
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Supported by the National Natural Science Fundation of China
Yi Qingming: born in Apr. 1938, Professor