Abstract
The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and 3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressed amount reached maxium in 4 h with IPTG induction.
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References
Kool M, Vlak JM. The structural and functional organization of theAutographa californica nuclear polyhedrosis virus Genome.Arch virol, 1993,130: 1–16
Ayers MD, Howard SC, Kuzio J. The complete DNA sequence ofAutogrpha californica nuclear polyhedrosis Virus.Virol, 1994,202: 586–605
Blissard GW, Quant-russell RL, Rohrmann GF,et al. Nucleotide sequence, transcriptional mapping, and temporal expression of the gene encoding p39, a major structural protein of the multicapsid nuclear polyhedrosis virus ofOrgyia pseudotsugata.Virol. 1989,169: 354–362
Chariton CA, Volkman LZ. Pentration ofAutographa californica nuclear polyhedrosis virus nucleo capsids into IPLB Sf21 cells Induces Actin cable formation.Virol, 1993,197: 245–254
Slack JM, Kuzio J, Faulkner P. Characterization of V-cath, a cathepsin L-like proteinase expressed by the baculovirusAutographa californica nuclear polyhedrosis Virus.J Genvirol, 1995,76: 1091–1098
Sambrook J, Fritsch EF, Maniatis TMolecular Cloning, A Laboratory Manual (2ed), New York: Cold Spring Harbor Press, 1989
Sanger F, Nicklen S, Coulsin AR. DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci USA 1977,74:5463
Rohrmann G F. Baculovirus structural proteins.J Gen virol, 1992,73: 749–761
Todd J W, Passarelli A L, Lu A,et al. Factors regulating baculovirus late and very late gene expression in transient-expression assays.J Virol, 1996,70: 2307–2317
Yue Lili, Qi Tipeng, Lin Hong,et al. The fusion expression of green fluoresent protein and HCV core antigene inEscherichia coli cells.Chinese Journal of Biotechnology, 1997,13(4): 368–374 (in Chinese)
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Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee
Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938)
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Deli, L., Xiaojie, S., Yipeng, Q. et al. Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli . Wuhan Univ. J. Nat. Sci. 3, 108–112 (1998). https://doi.org/10.1007/BF02827528
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DOI: https://doi.org/10.1007/BF02827528