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Glycosphingolipids in human pancreatic juice

Unique Fatty Acid Compositions of Glucosylceramides and Lactosylceramides

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Summary

Glycosphingolipids in human pancreatic juice were isolated and purified by DEAE and silica gel column chromatographs, and further by HPLC on silica gel and reversed-phase columns. The structures of the glycosphingolipids were determined to be glucosylceramides and lactosylceramides by means of fast atom bombardment mass spectrometry,1H-NMR spectroscopy, and component analysis involving GLC-mass spectrometry. The ceramide portions of the glucosylceramides consist of palmitic, tetracosanoic, and α-hydroxy tetracosanoic fatty acids, and d18:1 and t18:0 sphingosines. The ceramide portions of the lactosylceramides consist of palmitic, tetracosanoic, tetracosenoic, and tetracosadienoic fatty acids, and d18:1 sphingosine. The fatty acid compositions are different from the free fatty acid compositions of serum and pancreatic juice. The predominance of saturated, unsaturated, and hydroxy tetracosanoic fatty acids is quite unique, and the possibility that these glycosphingolipids may play a specific role in pancreatic juice deserves consideration. These glycosphingolipids were documented to be major components of the pancreatic juice from 15 patients with a variety of pancreatic diseases.

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Abbreviations

CDH:

ceramide dihexoside

CMH:

ceramide monohexoside

CTH:

ceramide trihexoside

GLC:

gas-liquid chromatography

GM3:

NeuAc-Gal-Glc-Cer

HPLC:

high-performance liquid chromatography

1H-NMR:

proton nuclear magnetic resonance

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Authors and Affiliations

  1. Department of Surgery II, Yamaguchi University School of Medicine, Yamaguchi

    Yuichiro Hamanaka & Takashi Suzuki

  2. Department of Dermatology, Yamaguchi, Rosai Hospital, Yamaguchi

    Sumiko Hamanaka

  3. Department of Internal Medicine, Yamaguchi, Rosai Hospital, Yamaguchi

    Toshio Kaneko

  4. Department of Membrane Biochemistry, The Tokyo Metropolitan Institute of Medical Science, Tokyo

    Minoru Suzuki & Akemi Suzuki

Authors
  1. Yuichiro Hamanaka
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  2. Sumiko Hamanaka
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  3. Toshio Kaneko
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  4. Takashi Suzuki
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  5. Minoru Suzuki
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  6. Akemi Suzuki
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Hamanaka, Y., Hamanaka, S., Kaneko, T. et al. Glycosphingolipids in human pancreatic juice. Int J Pancreatol 18, 33–39 (1995). https://doi.org/10.1007/BF02825419

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  • DOI: https://doi.org/10.1007/BF02825419

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