Skip to main content
Log in

Genetic engineering of plant signal transduction mechanisms

  • Review
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

Signal transduction is used by plants to coordinate their development and to sense and respond to fluctuations in their surroundings. Of prime importance is the ability to defend against pathogens and other environmental hazards such as cold temperatures, drought or wounding. Many transduction pathways are now characterized and the underlying genes are known. This suggests an obvious question—can we engineer signal transduction mechanisms for plant improvement? We address this question by presenting a rationale for an engineering approach and by discussing results from recent attempts to apply this approach. Calmodulin-like domain protein kinase (CDPK) and mitogen-activated protein kinase (MAPK) pathways are used as primary examples. New technology that will aid these efforts is also covered.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Aharon GS, Gelli A, Snedden WA and Blumwald E (1998) Activation of a plant plasma membrane Ca2+ channel by TGα1, a heterotrimetic G protein α-subunit homologue. FEBS Lett 424: 17–21.

    Article  PubMed  CAS  Google Scholar 

  • Baulcombe DC (1999) Fast forward genetics based on virus-induced gene silencing. Curr Opinion Plant Biol 2: 109–113.

    Article  CAS  Google Scholar 

  • Becraft PW (1998) Receptor kinases in plant development. Trends Plant Sci 3: 384–388.

    Article  Google Scholar 

  • Blumwald E, Aharon GS and Lam CHB (1998) Early signal transduction pathways in plant-pathogen interactions. Trends Plant Sci 3: 342–346.

    Article  Google Scholar 

  • Bögre L, Ligterink W, Meskiene I, Barker PJ, Heberle-Bors E, Huskisson NS and Hirt H (1997) Wounding induces the rapid and transient activation of a specific MAP kinase pathways. Plant Cell 9: 75–83.

    PubMed  Google Scholar 

  • Bouchez D and Höfte H (1998) Functional genomics in plants. Plant Physiol 118: 725–732.

    Article  PubMed  CAS  Google Scholar 

  • Fang YW, MaCool D, Heppard E, Hainey C and Miao GH (2000) Elucidation of host defense signal transduction pathways using the high throughput yeast two-hybrid screen. Plant and Animal Genome VIII Conference, San Diego, CA, USA January 9–12, 2000.

  • Gadella TWJ, van der Krogt GNM and Bisseling T (1999) GFP-based FRET microscopy in living plant cells. Trends Plant Sci 4: 287–291.

    Article  PubMed  Google Scholar 

  • Gelli A, Higgins VJ and Blumwald E (1997) Activation of plant plasma membrane Ca2+-permeable channels by race-specific fungal elicitors. Plant Physiol 113: 269–279.

    PubMed  CAS  Google Scholar 

  • Gordon GW et al. (1998) Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy. Biophys J 74: 2702–2713.

    Article  PubMed  CAS  Google Scholar 

  • Gupta R, Huang YF, Kieber J and Luan S (1998) Identification of a dual-specificity protein phosphatase that inactivates a MAP kinase fromArabidopsis. Plant J 16: 581–589.

    Article  PubMed  CAS  Google Scholar 

  • Higgins VJ, Lu H, Xing T, Gelli A and Blumwald E (1998) The gene-for-gene concept and beyond: interactions and signals. Can J Plant Pathol 20: 150–157.

    Article  CAS  Google Scholar 

  • Hirt H (1997) Multiple roles of MAP kinases in plant signal transduction. Trends Plant Sci 2: 11–15.

    Article  Google Scholar 

  • Hirt H (2000a) Connecting oxidative stress, auxin, and cell cycle regulation through a plant mitogen-activated protein kinase pathway. Proc Natl Acad Sci USA 97: 2405–2407.

    Article  PubMed  CAS  Google Scholar 

  • Hirt H (2000b) MAP kinase pathways: Molecular plug and play chips of cellular bio-informatics. 6th International Congress of Plant Molecular Biology. Quebec, Canada, June 18–24, 2000.

  • Huang JF, Teyton L and Harper JF (1996) Activation of a Ca2+-dependent protein kinase involved intramolecular binding of a calmodulin-like regulatory domain. Biochem 35: 13222–13230.

    Article  CAS  Google Scholar 

  • Jonak C, Kiegerl S, Ligterink W, Barker PJ, Huskisson NS and Hirt H (1996) Stress signaling in plants: a mitogen-activated protein kinase pathway is activated by cold and drought. Proc Natl Acad Sci USA 93: 11274–11279.

    Article  PubMed  CAS  Google Scholar 

  • Kovtun Y, Chiu WL, Zeng W and Sheen J (1998) Suppression of auxin signal transduction by a MAPK cascade in higher plants. Nature 395: 716–720.

    Article  PubMed  CAS  Google Scholar 

  • Kovtun Y, Chiu WL, Tena G and Sheen J (2000) Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc Natl Acad Sci USA 97: 2940–2945.

    Article  PubMed  CAS  Google Scholar 

  • Ligterink W, Kroj T, Nieden UZ, Hirt H and Scheel D (1997) Receptor-mediated activation of a MAP kinase in pathogen defense of plants. Science 276: 2054–2057.

    Article  PubMed  CAS  Google Scholar 

  • Miyawaki A et al. (1997). Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulins. Nature 388: 882–887.

    Article  PubMed  CAS  Google Scholar 

  • Romeis T, Piedras P, Zhang S, Klessig DF, Hirt H and Jones JDG (1999) Rapid Avr9- and Cf-9-dependent activation of MAP kinases in tobacco cell cultures and leaves: convergence of resistance gene, elicitor, wound, and salicylate responses. Plant Cell 11: 273–287.

    PubMed  CAS  Google Scholar 

  • Romeis T, Piedras P and Jones JDG (2000) Resistance gene-dependent activation of a calcium-dependent protein kinase in the plant defense response. Plant Cell 12: 803–815.

    PubMed  CAS  Google Scholar 

  • Saijo Y, Kinoshita N, Hata S, Kyozuka J, Shimamoto K and Izui K (2000) Overexpression of a single calcium-dependent protein kinase confers both cold- and salt/drought tolerance on rice plants. Plant J 23: 319–328.

    Article  PubMed  CAS  Google Scholar 

  • Schlessinger J (2000) Novel fluorescent approaches for studying cell signaling in single cells. Nature Biotech 18: 262–263.

    Article  CAS  Google Scholar 

  • Segal AW and Abo A (1993) The biochemical basis of the NADPH oxidase of phagocytes. Trends Biochem Sci 18: 43–47.

    Article  PubMed  CAS  Google Scholar 

  • Sheen J (1996) Ca2+-dependent protein kinase and stress signal transduction. Science 274: 1900–1902.

    Article  PubMed  CAS  Google Scholar 

  • Somerville S (2000) Microarray experiments in the study of plant-pathogen interactions and their limitations. 6th International Congress of Plant Molecular Biology. Quebec, Canada, June 18–24, 2000.

  • Suzuki K and Shinshi H (1995) Transient activation and tyrosine phosphorylation of a protein kinase in tobacco cells treated with a fungal elicitor. Plant Cell 7: 639–647.

    PubMed  CAS  Google Scholar 

  • Uetz P et al. (2000) A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature 403: 623–627.

    Article  PubMed  CAS  Google Scholar 

  • Usami S, Banno H, Ito Y, Nishihama R and Machida Y (1995) Cutting activates a 46-kilodalton protein kinase in plants. Proc Natl Acad Sci USA 92: 8660–8664.

    Article  PubMed  CAS  Google Scholar 

  • Wilson I, Vogel J and Somerville S (1997) Signaling pathways: A common theme in plants and animals? Current Biol 7: R175-R178.

    Article  CAS  Google Scholar 

  • Xing T (1998) Bioinformatics and its impact on plant science. Trends Plant Sci 3: 450.

    Article  Google Scholar 

  • Xing T (2000) Enhancement of plant disease resistance through manipulation of MAP kinase pathway. 6th International Congress of Plant Molecular Biology. Quebec, Canada, June 18–24, 2000.

  • Xing T, Higgins VJ and Blumwald E (1996) Regulation of plant defense response to fungal pathogens: two types of protein kinases in the reversible phosphorylation of the host plasma membranes H+-ATPase. Plant Cell 8: 555–564.

    PubMed  CAS  Google Scholar 

  • Xing T, Higgins VJ and Blumwald E (1997a) Race-specific elicitors ofCladosporium fulvum promote translocation of cytosolic components of NADPH oxidase to the plasma membrane of tomato cells. Plant Cell 9: 249–259.

    PubMed  CAS  Google Scholar 

  • Xing T, Higgins VJ and Blumwald E (1997b) Identification of G proteins mediating fungal elicitor-induced dephosphorylation of host plasma membrane H+-ATPase. J Exp Bot 48: 229–237.

    Article  CAS  Google Scholar 

  • Zhang S and Klessig DF (1997) Salicylic acid activates a 48-kD MAP kinase in tobacco. Plant Cell 9: 809–824.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Tim Xing.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xing, T., Jordan, M. Genetic engineering of plant signal transduction mechanisms. Plant Mol Biol Rep 18, 309–318 (2000). https://doi.org/10.1007/BF02825058

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02825058

Key words

Navigation